1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 74, Issue 5

sp. nov. and sp. nov. isolated from lichen (Sw.) Nyl. No Access

Abstract

Two novel actinobacteria, designated as LP05-1 and LP11, were isolated from the lichen (Sw.) Nyl. collected in Bangkok, Thailand. Genotypic and phenotypic analyses revealed that both strains represented members of the genus . The 16S rRNA gene of LP05-1 showed the highest similarity to the genome of (98.41 %), while the 16S rRNA gene of LP11 was most similar to that of (98.93 %). The major menaquinones in LP05-1 were MK-9(H), MK-9(H), MK-9(H) and MK-9(H), and in LP11, they were MK-9(H) and MK-9(H). Both strains exhibited the major fatty acids iso-C, anteiso-C, iso-C and anteiso-C, with LP05-1 also possessing iso-C. The polar lipids of LP05-1 included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified lipid, while those of LP11 consisted of phosphatidylethanolamine, -phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified aminolipid and an unidentified glycolipid. The digital DNA–DNA hybridisation (dDDH) and average nucleotide identity (ANI) values indicated that both strains are distinct from each other with values below 70 and 95 %, respectively. dDDH, ANI by (ANIb) and ANI by MUMmer (ANIm) values between LP05-1 and its closely related type strains were 26.07–26.80 %, 81.24–82.01 % and 86.82–86.96 %, respectively, while those for LP11 and its closely related type strains were 30.70–31.70 %, 84.09–85.31 % and 88.02–88.39 %, respectively. The results of the taxonomic investigation, including dDDH and ANI values, indicate that LP05-1 and LP11 are novel type strains of two novel species within the genus . The names proposed are sp. nov. for strain LP05-1 (=TBRC 15494, =NBRC 115434) and sp. nov. for strain LP11 (=TBRC 15493, =NBRC 115421).

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  • Accepted:
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Keyword(s): Actinobacteria , lichen , Pyxine , Streptomyces and taxonomy
Funding
This study was supported by the:
  • The 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund)
    • Principle Award Recipient: AchirayaSomphong
  • The 100th Anniversary Chulalongkorn University Fund for Doctoral Scholarship
    • Principle Award Recipient: AchirayaSomphong
© 2024 The Authors
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Streptomyces pyxinae sp. nov. and Streptomyces pyxinicus sp. nov. isolated from lichen Pyxine cocoes (Sw.) Nyl.
Int J Syst Evol Microbiol 74, 006364 (2024); https://doi.org/10.1099/ijsem.0.006364
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