1887

Abstract

Summary

Following SY 40 infection of monkey kidney (CV-1) cells, DNA polymerase activity was induced. Enzyme formation was inhibited by cycloheximide, but not by l- --arabinofuranosylcytosine. Enhanced levels of DNA polymerase activity were also found in mouse kidney cell lines which had been transformed by SY 40.

The DNA polymerases from uninfected and SV 40-infected CV-1 cells were partially purified and compared. Both enzymes required denatured DNA as primer and were similar with respect to pH optima, amount of primer required for maximal activity, kinetics of thermal denaturation, and sensitivity to inhibition by ammonium sulphate and sodium dodecyl sulphate. However, the Michaelis constant () for [H]thymidine 5′-triphosphate of the SV 40-induced enzyme was about half that of the enzyme from uninfected cells. The value of DNA polymerase partially purified from SV 40- transformed mouse cells was about the same as that of the enzyme from uninfected CV-1 cells.

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1967-04-01
2024-04-18
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References

  1. Andrews P. 1965; The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem. J 96:595
    [Google Scholar]
  2. Aposhian H. V., Kornberg A. 1962; Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of E. coli. A new enzyme. J. biol. Chem 237:519
    [Google Scholar]
  3. Dubbs D. R., Kit S. 1965; The effect of temperature on induction of deoxythymidine kinase activity by herpes simplex mutants. Virology 25:256
    [Google Scholar]
  4. Dulbecco R., Hartwell L. H., Vogt M. 1965; Induction of cellular DNA synthesis by polyoma virus. Proc. natn. Acad. Sci., U.S.A 53:403
    [Google Scholar]
  5. Frearson P. M., Kit S., Dubbs D. R. 1966; Induction of dihydrofolate reductase activity by SV40 and polyoma virus. Cancer Res 26:1653
    [Google Scholar]
  6. Green M., Piña M., Chagoya V. 1964; Biochemical studies on adenovirus multiplication. V. Enzymes of deoxyribonucleic acid synthesis in cells infected by adenovirus and vaccinia viruses. J. biol. Chem 239:1188
    [Google Scholar]
  7. Hamada C., Kamiya T., Kaplan A. S. 1966; Serological analysis of some enzymes present in pseudorabies virus-infected and noninfected cells. Virology 28:271
    [Google Scholar]
  8. Hartwell L. H., Vogt M., Dulbecco R. 1965; Induction of cellular DNA synthesis by polyoma virus. II. Increase in the rate of enzyme synthesis after infection with polyoma virus in mouse kidney cells. Virology 27:262
    [Google Scholar]
  9. Keir H. M., Subak-Sharpe H., Shedden W. I. H., Watson D., Wildy P. 1966; Immunological evidence for a specific DNA polymerase coded by herpes simplex virus. Virology 30:154
    [Google Scholar]
  10. Kit S. 1967; Enzyme inductions in cell cultures during productive infection and during abortive infection by papovavirus SV40. In Symposium on the Molecular Biology of Viruses Colter John. Ed. by New York: Academic Press, Inc; in the Press
    [Google Scholar]
  11. Kit S., Dubbs D. R. 1965; Properties of deoxythymidine kinase partially purified from noninfected mouse fibroblast cells. Virology 26:16
    [Google Scholar]
  12. Kit S., de Torres R. A., Dubbs D. R. 1966a; Arabinofuranosyl-cytosine-induced stimulation of thymidine kinase and deoxycytidylic deaminase activities of mammalian cultures. Cancer Res 26:1859
    [Google Scholar]
  13. Kit S., Dubbs D. R., Frearson P. M. 1966b; Enzymes of nucleic acid metabolism in cells infected with polyoma virus. Cancer Res 26:638
    [Google Scholar]
  14. Kit S., Dubbs D. R., Frearson P. M., Melnick J. L. 1966c; Enzyme induction in SV40-infected green monkey kidney cultures. Virology 29:69
    [Google Scholar]
  15. Kit S., Dubbs D. R., Piekarski L. J., de Torres R. A., Melnick J. L. 1966c; Acquisition of enzyme function by mouse kidney cells abortively infected with papovavirus SV40. Proc. natn. Acad. Sci., U.S.A 56:463
    [Google Scholar]
  16. Kit S., Dubbs D. R., Anken M. 1967a; Altered properties of thymidine kinase after infection of mouse fibroblast cells with herpes simplex virus. J. Virology in the Press
    [Google Scholar]
  17. Kit S., Melnick J. L., Dubbs D. R., Piekarski L. J., de Torres R. A. 1967b; Early enzyme synthesis in cell cultures infected with papovaviruses, SV 40 and polyoma. In Virus Directed Host Response Pollard Morris. Ed. by New York: Academic Press, Inc; in the Press
    [Google Scholar]
  18. Kit S, Dubbs D. R., de Torres R. A., Anken M. 1967c; Enzyme induction in simian adenovirus-infected green monkey kidney cultures. J. Virology in the Press
    [Google Scholar]
  19. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin-phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  20. Magee W. 1962; DNA polymerase and deoxyribonucleotide kinase activities in cells infected with vaccinia virus. Virology 17:604
    [Google Scholar]
  21. Magee W. E., Miller O. V. 1966; Evidence for the viral specific nature of the DNA-polymerase formed in cells infected with vaccinia virus. Fed. Proc 25:652
    [Google Scholar]
  22. Orr C. W. M., Herriott S. T., Bessman M. J. 1965; The enzymology of virus-infected bacteria. VII. A new deoxyribonucleic acid polymerase induced by bacteriophage T5. J. biol. Chem 240:4652
    [Google Scholar]
  23. Rapp F., Butel J. S., Feldman L. A., Kitahara T., Melnick J. L. 1965; Differential effects of inhibitors on the steps leading to the formation of SV40 tumor and virus antigens. J. exp. Med 121:935
    [Google Scholar]
  24. Sheinin R. 1966; Studies on thymidine kinase activity of mouse embryo cells infected with polyoma virus. Virology 28:47
    [Google Scholar]
  25. de Waard A., Paul A. V., Lehman I. R. 1965; The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5. Proc. natn. Acad. Sci. U.S.A 54:1241
    [Google Scholar]
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