1887

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Volume 1, Issue 3

The Restriction of Bacteriophage λ in Strain w Free

Abstract

Summary

strain w adsorbs phage very efficiently but the phage does not form plaques on this strain. In a very small fraction (10) of the infected cells the phage grows and produces small bursts of progeny phage also unable to form plaques on strain w. strain w is lysogenic for a temperate phage, w, related to phage 2. Non-restricting hosts for phage became restricting hosts when made lysogenic for w. When P-labelled adsorbed to restricting w lysogenic hosts, > 20% of the P become acid-soluble shortly after infection. No w specific modification was carried by the small number of Phages which escaped this restriction process. It is concluded that w controls a host-restriction mechanism but not a host-modification process, and in parallel with other examples of host-controlled restriction and modification can be represented as or °. mutants have been isolated which escape this restriction and which form plaques on strain w and w w lysogenic strains with an efficiency of 1·. With these mutants a w-specific host modification controlled by the genome of strain w was demonstrated. Mixed infection experiments with restricted and unrestricted showed that that restricted phage did not block the growth of the unrestricted mutant nor did the mutant permit the restricted phage to grow. In addition it was shown that obtained from bacteria mixedly infected with and was still unable to grow in restricting hosts and similarly obtained from mixedly infected bacteria still retained its ability to grow on restricting hosts. It is concluded that there is a nucleotide sequence in the DNA of phage which, when infects a restricting host, is specifically recognized by the restriction mechanism controlled by the w. The mutation to involves an alteration to this sequence such that it is no longer recognized by the restriction mechanism of the w.

Mutants of wwere isolated not restrictive for phage .

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1967
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1967-07-01
2024-04-24
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References

  1. Adams H. M. 1950; Methods for the study of bacterial viruses. Meth. med. Res 2:1
     [Google Scholar]
  2. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochem. biophys. Res. Commun 18:788
     [Google Scholar]
  3. Appleyard R. K. 1954; Segregation of new lysogenic types during growth of doubly lysogenic strain derived from Escherichia coli K-12. Genetics 39:440
     [Google Scholar]
  4. Arber W. 1960; Polylysogeny for bacteriophage lambda. Virology 11:250
     [Google Scholar]
  5. Arber W. 1965a; Host-controlled modification of bacteriophage. A. Rev. Microbiol 19:365
     [Google Scholar]
  6. Arber W. 1965b; Host specificity of DNA produced by Escherichia coli. V. The role of methionine in the production of host specificity. J. molec. Biol n:247
     [Google Scholar]
  7. Arber W., Dussoix D. 1962; Host specificity of DNA produced by Escherichia coli. I. Host controlled modification of bacteriophage γ. J. molec. Biol 5:18
     [Google Scholar]
  8. Arber W., Smith J. D. 1966; Host-controlled modification of phage and its correlation with specific methylation of desoxyribonucleotides. Int. Cong. Microbiol. IXth p. 5
     [Google Scholar]
  9. Arber W., Hattman S., Dussoix D. 1963; On the host-controlled modification of bacteriophage γ. Virology 21:30
     [Google Scholar]
  10. Bertani G. 1962; Multiple lysogeny from single infection. Virology 18:131
     [Google Scholar]
  11. Bertani G., Weigle J. J. 1953; Host controlled variation in bacterial viruses. J. Bact 65:113
     [Google Scholar]
  12. Colson C., Glover S. W., Symonds N., Stacey K. A. 1965; The location of the genes for host-controlled modification and restriction in Escherichia coli K-12. Genetics 52:1043
     [Google Scholar]
  13. Davis B. D. 1950; Studies on nutritionally deficient bacterial mutants isolated by means of penicillin. Experientia 6:41
     [Google Scholar]
  14. Dussoix D., Arber W. 1962; Host specificity of DNA produced by Escherichia coli. II. Control over acceptance of DNA from infecting phage. J. molec. Biol 5:37
     [Google Scholar]
  15. Eisen H. A., Fuerst C. R., Siminovitch L., Thomas R., Lambert L., Pereira da Silva L., Jacob F. 1966; Genetics and physiology of defective lysogeny in K-12 (γ): Studies of early mutants. Virology 30:224
     [Google Scholar]
  16. Fukasawa T. 1964a; The course of infection with abnormal bacteriophage T4 containing non-glucosylated DNA on Escherichia coli strains. J. molec. Biol 9:525
     [Google Scholar]
  17. Fukasawa T. 1964b; Localization of restricting function of E. coli strains to host-modified T-even phage. Fed. Proc. Fedn. Am. Soc. exp. Biol 23:292
     [Google Scholar]
  18. Glover S. W. 1962; Valine resistant mutants of Escherichia coli K-12. Genet. Res 3:448
     [Google Scholar]
  19. Glover S. W., Aronovitch J. 1967; Mutants of bacteriophage lambda able to grow on the restricting host Escherichia coli strain w. Genet. Res 9:129
     [Google Scholar]
  20. Glover S. W., Kerszman G. 1967; The properties of a temperate bacteriophage wø isolated from Escherichia coli strain w. Genet. Res 9:135
     [Google Scholar]
  21. Glover S. W., Schell J., Symonds N., Stacey K. A. 1963; The control of host-induced modification by phage PI. Genet. Res 4:138
     [Google Scholar]
  22. Gold M., Hausmann R., Maitra V., Hurwitz J. 1964; The enzymatic methylation of RNA and DNA. VIII. Effects of bacteriophage infection on the activity of the methylating enzymes. Proc. natn. Acad. Sci. U.S.A 52:292
     [Google Scholar]
  23. Jacob F., Wollman E. L. 1954; Étude génétique d’un bactériophage tempéré d’Escherichia coli. I. Le systéme génétique du bactériophage γ. Annls Inst. Pasteur, Paris 87:653
     [Google Scholar]
  24. Kelly B. 1963; Localization of P2 prophage in two strains of Escherichia coli. Virology 19:32
     [Google Scholar]
  25. Klein A. 1965; Wirtskontrollierte modifikation. Z. VererbLehre 96:324
     [Google Scholar]
  26. Klein A., Sauerbier W. 1965; Role of methylation in host-controlled modification of phage T 1. Biochem. biophys. Res. Commun 18:440
     [Google Scholar]
  27. Lederberg S. 1966; Genetics of host-controlled restriction and modification of deoxyribonucleic acid in Escherichia coli. J. Bact 91:1029
     [Google Scholar]
  28. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage PI. Virology 1:190
     [Google Scholar]
  29. Molholt B., Fraser D. 1965; Reversal of the restriction for host-modified T2 and T4 DNA upon conversion of non-permissive Escherichia coli to spheroplasts. Biochem. biophys. Res. Commun 19:571
     [Google Scholar]
  30. Paigen K., Weinfeld H. 1963; Co-operative infection by host-modified lambda phage. Virology 19:565
     [Google Scholar]
  31. Pizer L. I., Miovic M., Pylkas L. 1967; The effect of prophage w on the propagation of T2 and T4. J. Bact in the Press
    [Google Scholar]
  32. Schell J., Glover S. W. 1966a; On the localization of a factor responsible for host-controlled restriction in Escherichia coli K(P I). Genet. Res 7:277
     [Google Scholar]
  33. Schell J., Glover S. W. 1966b; The effect of various physiological conditions on host-controlled restriction in Escherichia coli K(PI). Genet. Res 7:273
     [Google Scholar]
  34. Schell J., Glover S. W. 1966c; The effect of heat on host-controlled restriction of phage A in Escherichia coli K(P I). J. gen. Microbiol 45:61
     [Google Scholar]
  35. Smith H. S., Pizer L. I. 1966; Abortive infection of Escherichia coli strain w by T2. Bact. Proc 25:127
     [Google Scholar]
  36. Stent G. S., Fuerst C. R. 1955; Inactivation of bacteriophages by decay of incorporated radioactive phosphorus. J. gen. Physiol 38:441
     [Google Scholar]
  37. Wood W. B. 1966; Host specificity of DNA produced by Escherichia coli: Bacterial mutations affecting the restriction and modification of DNA. J. molec. Biol 16:118
     [Google Scholar]
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The Restriction of Bacteriophage λ in Escherichia coli Strain w
J. Gen. Virol. 1, 333 (1967); https://doi.org/10.1099/0022-1317-1-3-333
/content/journal/jgv/10.1099/0022-1317-1-3-333
/content/journal/jgv/10.1099/0022-1317-1-3-333
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