1887

Abstract

SUMMARY

Polyoma virus demonstrated a requirement for all of the individual amino acids of Eagle’s minimal essential medium; however, arginine deprivation inhibited virus synthesis to the greatest extent. Early recovery of virus synthesis upon addition of arginine to an arginine-deprived infection indicated that arginine deprivation possibly inhibited a late step of virus synthesis. Arginine deprivation inhibited polyoma virus DNA synthesis by 60%, whereas plaque-forming activity was inhibited approximately 90% during the same period. Arginine deprivation did not affect the number of cells synthesizing structural antigens; however, the encapsidation of virus DNA, dependent upon the synthesis of all structural proteins necessary for virus maturation, was inhibited approximately 90%. A portion of the particles synthesized in arginine-deprived cultures was susceptible to nuclease digestion suggesting incomplete assembly.

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/content/journal/jgv/10.1099/0022-1317-10-1-53
1971-01-01
2024-04-19
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References

  1. Becker Y., Olshevsky U., Levitt J. 1967; The role of arginine in the replication of herpes simplex virus. Journal of General Virology 1:471
    [Google Scholar]
  2. Consigli R. A., Minocha H. C., Abo-Ahmed H. 1966; Multiplication of polyoma virus II. Source of constituents for viral deoxyribonucleic acid and protein synthesis. Journal of Bacteriology 92:789
    [Google Scholar]
  3. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science, New York 130:432
    [Google Scholar]
  4. Fine R., Moss M., Murakami W. T. 1968; Protein composition of polyoma virus. Journal of Molecular Biology 36:167
    [Google Scholar]
  5. Goldblum N., Ravid Z., Becker Y. 1968; Effect of withdrawal of arginine and other amino acids on the synthesis of tumour and viral antigens of SV 40 virus. Journal of General Virology 3:143
    [Google Scholar]
  6. Hirt B. 1967; Selective extraction of polyoma virus DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365
    [Google Scholar]
  7. Inglis V. B. 1968; Requirement of arginine for the replication of herpes virus. Journal of General Virology 3:9
    [Google Scholar]
  8. Jeney E., Gönczöl E., Vaczi L. 1967; Replication of herpes simplex virus in arginine-free media. I. Effect of arginine-deficiency in different tissue culture cells. Acta microbiologica Academiae scientiarum hungaricae 14:31
    [Google Scholar]
  9. Mäntyjärvi R., Russell W. C. 1969; Studies on DNA synthesis in cells infected with adenovirus. Journal of General Virology 5:339
    [Google Scholar]
  10. Murakami W. T., Fine R., Harrington M. R., Sassar Z. B. 1968; Properties and amino acid composition of polyoma virus purified by zonal ultracentrifugation. Journal of Molecular Biology 36:153
    [Google Scholar]
  11. Perry J. T., To C. M., Consigli R. A. 1969; Alkaline degradation of polyoma virus. Journal of General Virology 4:403
    [Google Scholar]
  12. Radloff R., Bauer W., Vinograd S. 1967; A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 57:1514
    [Google Scholar]
  13. Rouse H. C., Schlesinger R. W. 1967; An arginine-dependent step in the maturation of type 2 adenovirus. Virology 33:513
    [Google Scholar]
  14. Russell W. C., Becker Y. 1968; A maturation factor for adenovirus. Virology 35:18
    [Google Scholar]
  15. Spring S. B., Roizman B., Spear P. G. 1969; Selective failure of protein synthesis in herpes virus-infected cells deprived of arginine. Virology 38:710
    [Google Scholar]
  16. Tankersley R. W. 1964; Amino acid requirements of herpes simplex virus in human cells. Journal of Bacteriology 87:609
    [Google Scholar]
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