RT Journal Article SR Electronic(1) A1 Schild, G. C. A1 Henry-Aymard, Michele A1 Pereira, H. G.YR 1972 T1 A Quantitative, Single-Radial-Diffusion Test for Immunological Studies with Influenza Virus JF Journal of General Virology, VO 16 IS 2 SP 231 OP 236 DO https://doi.org/10.1099/0022-1317-16-2-231 PB Microbiology Society, SN 1465-2099, AB Antibodies for influenza haemagglutinin and neuraminidase are assayed routinely in haemagglutination-inhibition (Hirst, 1942) and neuraminidase-inhibition (Ada, Lind & Laver, 1963) tests which involve reaction systems of three components. More recently, influenza antibodies have been detected by gel immuno-double-diffusion tests with influenza viruses disrupted by detergents (Styk & Hanna, 1966; Schild & Pereira, 1969). Although the latter method has the advantage that it detects antibodies for each of the major antigens of the virus in a single test system (Schild et al. 1972a; Schild, 1972) the quantitation of antibody is not readily achieved. In the present report we describe a quantitative, single-radial-diffusion test which is a sensitive, rapid and convenient method for the estimation of antibodies to the haemagglutinin and neuraminidase antigens of the influenza virus. A unique feature of this test is that only one component, the antibody, diffuses during the test; the other component, the antigen in the form of intact influenza virus particles, does not diffuse., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-16-2-231