1887

Abstract

SUMMARY

Rat embryo cells have been transformed by temperature-sensitive mutants of herpes simplex type 2 and by wild-type virus of herpes simplex types 1 and 2. Transformation was scored using the morphological criterion of focus formation and the cell changes that lead to the final focus are described.

Herpes specified antigens can be detected in at least 50% of these transformed rat embryo cells by immunofluorescence studies on unfixed material.

Transformation experiments using hamster embryo cells and HSV-2 strain 333 are also described. These transformed cells are shown to be tumourigenic in newborn hamsters. Tumour cells in turn, as a single cell suspension, produce fresh tumours in animals up to 2 weeks old with almost 100% efficiency. Tumour cells also continue to retain the herpes specific antigens.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-24-1-143
1974-07-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/24/1/JV0240010143.html?itemId=/content/journal/jgv/10.1099/0022-1317-24-1-143&mimeType=html&fmt=ahah

References

  1. Brown S. M., Ritchie D. A., Subak-Sharpe J. H. 1973; Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map. Journal of General Virology 18:329–346
    [Google Scholar]
  2. Darai O., Munk K. 1973; Human embryonic lung cells abortively infected with herpes virus hominis type 2 show some properties of cell transformation. Nature New Biology 241:268–269
    [Google Scholar]
  3. Davidson R. L., Adelstein S. J., Oxman M. N. 1973; Herpes simplex virus as a source of thymidine kinase for thymidine kinase deficient mouse cells: suppression and reactivation of the viral enzyme. Proceedings of the National Academy of Sciences of the United States of America 70:1912–1916
    [Google Scholar]
  4. Doller E., Duff R., Rapp F. 1973; Resistance of hamster cells transformed by herpes simplex virus type 2 to superinfection by herpes simplex viruses. Intervirology 1:154–167
    [Google Scholar]
  5. Duff R., Rapp F. 1971; Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultra-violet irradiated herpes simplex virus type 2. Journal of Virology 8:469–477
    [Google Scholar]
  6. Duff R., Rapp F. 1973; Oncogenic transformation of hamster embryo cells after exposure to inactivated herpes simplex virus type 1. Journal of Virology 12:209–217
    [Google Scholar]
  7. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99:167–182
    [Google Scholar]
  8. Halliburton I. W., Timbury M. C. 1973; Characterisation of temperature-sensitive mutants of herpes simplex virus type 2. Growth and DNA synthesis. Virology 54:60–68
    [Google Scholar]
  9. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  10. Margalith M., Volk-Fuchs R., Goldblum N. 1969; Transformation of BSC-1 cells following chronic infection with SV40. Journal of General Virology 5:321–327
    [Google Scholar]
  11. Mechie M. 1974; A biological and biochemical characterisation of ts mutants of herpes simplex virus type 1. Ph.D. Thesis University of Glasgow;
    [Google Scholar]
  12. Munyon D., Kraiselburd W., Davies E., Mann J. 1971; Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus. Journal of Virology 7:813–820
    [Google Scholar]
  13. Timbury M. C. 1971; Temperature sensitive mutants of herpes simplex virus type 2. Journal of General Virology 13:373–376
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-24-1-143
Loading
/content/journal/jgv/10.1099/0022-1317-24-1-143
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error