@article{mbs:/content/journal/jgv/10.1099/0022-1317-25-1-83, author = "Noble-Harvey, Jane and Lonberg-Holm, K.", title = "Sequential Steps in Attachment of Human Rhinovirus Type 2 to HeLa Cells", journal= "Journal of General Virology", year = "1974", volume = "25", number = "1", pages = "83-91", doi = "https://doi.org/10.1099/0022-1317-25-1-83", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-25-1-83", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY When human rhinovirus type 2 attaches to HeLa cells, it first forms a reversible complex which can be dissociated by addition of EDTA to the medium. After prolonged incubation at 25 °C, most of the cell-associated virus progresses to an irreversible complex from which it cannot be eluted by EDTA, although some noninfectious material elutes spontaneously. The addition of a sufficient concentration of SDS to block cell-mediated eclipse of the virus does not block the formation of the irreversible complex at 25 °C, while virus adsorbed and incubated at 0 °C remains reversibly bound. The number of receptor sites per cell is not influenced by SDS, but at 0 °C cells appear to have fewer receptor sites than at physiological temperature.", }