RT Journal Article SR Electronic(1) A1 Noble-Harvey, Jane A1 Lonberg-Holm, K.YR 1974 T1 Sequential Steps in Attachment of Human Rhinovirus Type 2 to HeLa Cells JF Journal of General Virology, VO 25 IS 1 SP 83 OP 91 DO https://doi.org/10.1099/0022-1317-25-1-83 PB Microbiology Society, SN 1465-2099, AB SUMMARY When human rhinovirus type 2 attaches to HeLa cells, it first forms a reversible complex which can be dissociated by addition of EDTA to the medium. After prolonged incubation at 25 °C, most of the cell-associated virus progresses to an irreversible complex from which it cannot be eluted by EDTA, although some noninfectious material elutes spontaneously. The addition of a sufficient concentration of SDS to block cell-mediated eclipse of the virus does not block the formation of the irreversible complex at 25 °C, while virus adsorbed and incubated at 0 °C remains reversibly bound. The number of receptor sites per cell is not influenced by SDS, but at 0 °C cells appear to have fewer receptor sites than at physiological temperature., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-25-1-83