f Cauliflower Mosaic Virus: an Improved Purification Procedure and Some Properties of the Virus Particles
- Authors: R. Hull*, R. J. Shepherd, J. D. Harvey
- J. Gen. Virol., April 1976 31: 93-100, doi: 10.1099/0022-1317-31-1-93
- Subject: Articles
- Published Online:
A new procedure for the purification of cauliflower mosaic virus (CaMV) which involves the use of Triton X-100 and urea results in higher and more reliable yields. CaMV particles have a s 20, w of 208S, a D 20, w of 0.75 × 10-7 cm2/s, contain 17% nucleic acid and have a mol. wt. of 22.8 × 106. Virus precipitated by Mg2+, low pH or ethanol at moderate salt concentrations, or by polyethylene glycol, is not easily resuspended.
Present address: John Innes Institute, Colney Lane, Norwich, England.
© Society for General Microbiology 1976 | Published by the Microbiology Society
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