@article{mbs:/content/journal/jgv/10.1099/0022-1317-31-1-93, author = "Hull, R. and Shepherd, R. J. and Harvey, J. D.", title = "Cauliflower Mosaic Virus: an Improved Purification Procedure and Some Properties of the Virus Particles", journal= "Journal of General Virology", year = "1976", volume = "31", number = "1", pages = "93-100", doi = "https://doi.org/10.1099/0022-1317-31-1-93", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-31-1-93", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY A new procedure for the purification of cauliflower mosaic virus (CaMV) which involves the use of Triton X-100 and urea results in higher and more reliable yields. CaMV particles have a s 20, w of 208S, a D 20, w of 0µ75 × 10-7 cm2/s, contain 17% nucleic acid and have a mol. wt. of 22.8 × 106. Virus precipitated by Mg2+, low pH or ethanol at moderate salt concentrations, or by polyethylene glycol, is not easily resuspended.", }