@article{mbs:/content/journal/jgv/10.1099/0022-1317-36-1-59, author = "Graham, F. L. and Smiley, J. and Russell, W. C. and Nairn, R.", title = "Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5", journal= "Journal of General Virology", year = "1977", volume = "36", number = "1", pages = "59-72", doi = "https://doi.org/10.1099/0022-1317-36-1-59", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-36-1-59", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in other human cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.", }