1887

Abstract

SUMMARY

We have examined the matrix proteins of A/Okuda/57, A/Finland/4/74 and A/New Jersey/8/76 viruses and several recombinant strains by radioiodination of the purified polypeptides followed by tryptic peptide mapping. The method is rapid and requires only small amounts of material. Reproducible differences were detected between the matrix proteins of the above parents and allowed the origin of the matrix proteins of the recombinant viruses to be determined. The possible use of matrix protein identity as a marker in recombination work is discussed.

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1977-09-01
2024-04-20
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References

  1. Beare A. S., Craig J. W. 1976; Virulence for man of a human influenza A virus antigenically similar to ‘classical’ swine viruses. Lancet 11:4–5
    [Google Scholar]
  2. Brand C. M. 1972; Studies on the structural components of influenza viruses. Ph.D. Thesis Council for National Academic Awards;
    [Google Scholar]
  3. Brand C. M., Skehel J. J. 1972; Crystalline antigen from the influenza virus envelope. Nature New Biology 238:145–147
    [Google Scholar]
  4. Bray D., Brownlee S. M. 1973; Peptide mapping of proteins from acrylamide gels. Analytical Biochemistry 55:213–221
    [Google Scholar]
  5. Compans R. W., Klenk H.-D., Caliguiri L. A., Choppin P. W. 1970; Influenza virus proteins, i. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology 42:880–889
    [Google Scholar]
  6. Dimmock N. J., Carver A. S., Kennedy S. I. T., Lee M. R., Luscombe S. 1977; Internal proteins of influenza virus: 35S-methionine peptide maps as genetic markers. Journal of General Virology 36:403–413
    [Google Scholar]
  7. Fazekas De St Groth S., White D. O. 1958; An improved assay for the infectivity of influenza viruses. Journal of Hygiene 56:151–162
    [Google Scholar]
  8. Laver W. G., Downie J. C. 1976; Influenza virus recombination. I. Matrix protein markers and segregation during mixed infections. Virology 70:105–117
    [Google Scholar]
  9. Lief F. S., Fabiyi A., Henle W. 1958; Antigenic analyses of influenza viruses by complement fixation. 1. The production of antibodies to the soluble antigen in guinea pigs. Journal of Immunology 80:53–65
    [Google Scholar]
  10. Mccahon D., Beare A. S., Stealey V. 1976; The production of live attenuated influenza A strains by recombination with A/Okuda/57 (H2N2). Postgraduate Medical Journal 52:389–394
    [Google Scholar]
  11. Rott R., Orlich M., Scholtissek C. 1976; Attenuation of pathogenicity of fowl plague virus by recombination with other influenza A viruses nonpathogenic for fowl: nonexclusive dependence of pathogenicity on haemagglutinin and neuraminidase of the virus. Journal of Virology 19:54–60
    [Google Scholar]
  12. Schild G. C. 1972; Evidence for a new type-specific structural antigen of the influenza virus particle. Journal of General Virology 15:99–103
    [Google Scholar]
  13. Schulze I. T. 1970; The structure of influenza virus. 1. The polypeptides of the virion. Virology 42:890–904
    [Google Scholar]
  14. Schulze I. T. 1973; Structure of the influenza virion. Advances in Virus Research 18:1–55 London and New York: Academic Press;
    [Google Scholar]
  15. Skehel J. J. 1971; The characterization of subviral particles derived from influenza virus. Virology 44:409–417
    [Google Scholar]
  16. Skehel J. J., Schild G. C. 1971; The polypeptide composition of influenza A viruses. Virology 44:396–408
    [Google Scholar]
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