1887

Abstract

SUMMARY

Capsid types present in hepatocyte nuclei of Syrian hamsters infected with the animal strain of equine herpesvirus type 1 (EHV-1H) were studied and characterized. Three capsid species were isolated: L capsids ( = 1.23 g/ml) which are devoid of a core structure and appear to be empty shells, I capsids ( = 1.24 g/ml) which contain an electron-lucent, cross-shaped, immature core, and H capsids ( = 1.25 g/ml) which contain an electron-dense, mature core structure. All three capsids first appeared at approx. 6 h post inoculation and were present in a ratio of approx. 10:87:3 (L:I:H) at all times during infection. Analysis of the polypeptide and amino acid compositions of certain species indicated that these capsids are identical to L, I and H capsids isolated from cell cultures infected with the culture tissue strain of EHV-1. These findings support the model (O’Callaghan & Randall, 1976) that I capsids are a major precursor of mature capsids and play a major role in the maturation of this herpesvirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-37-3-585
1977-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/37/3/JV0370030585.html?itemId=/content/journal/jgv/10.1099/0022-1317-37-3-585&mimeType=html&fmt=ahah

References

  1. Abodeely R. A., Lawson L. A., Randall C. C. 1970; Morphology and entry of enveloped and deenveloped equine abortion (Herpes) virus. Journal of Virology 5:513–523
    [Google Scholar]
  2. Allen G. P., Bryans J. T. 1976; Cell-free synthesis of equine herpesvirus type 3 nucleocapsid polypeptides. Virology 69:751–762
    [Google Scholar]
  3. Allen G. P., O’Callaghan D. J., Randall C. C. 1977; Purification and characterization of equine herpesvirus-induced DNA polymerase. Virology 76:395–408
    [Google Scholar]
  4. Darlington R. W., Moss L. H. 1968; Herpesvirus envelopment. Journal of Virology 2:48–55
    [Google Scholar]
  5. Darlington R. W., Randall C. C. 1963; The nucleic acid content of equine abortion virus. Virology 19:322–327
    [Google Scholar]
  6. Gibson W., Roizman B. 1972; Proteins specified by herpes simplex virus. VII. Characterization and composition of multiple capsid forms of sub types 1 and 2. Journal of Virology 10:1044–1052
    [Google Scholar]
  7. Gibson W., Roizman B. 1974; Proteins specified by herpes simplex virus. X. Staining and radiolabelling properties of B-capsid and virion proteins in polyacrylamide gels. Journal of Virology 13:155–165
    [Google Scholar]
  8. Henry B. E., Newcomb W. W., Randall C. C., O’Callaghan D. J. 1977Abstracts of the Annual Meeting of the American Society for Microbiology New Orleans: p 314
  9. Kemp M. C., Cohen J. C., O’Callaghan D. J., Randall C. C. 1975; Equine herpesvirus induced alterations in nuclear RNA and DNA polymerase activities. Virology 68:467–482
    [Google Scholar]
  10. Kemp M. C., Perdue M. L., Rogers H. W., O’Callaghan D. J., Randall C. C. 1974; Structural polypeptides of the hamster strain of equine herpes virus type 1: products associated with purification. Virology 61:361–375
    [Google Scholar]
  11. O’Callaghan D. J., Allen G. P., Randall C. C. 1977; Structure and replication of the equine herpesviruses. Proceedings of the 4th International Conference on Equine Infectious Diseases, Lyon, France Basel: Karger; (in the press)
    [Google Scholar]
  12. O’Callaghan D. J., Randall C. C. 1976; Molecular anatomy of herpesviruses: recent studies. In Progress in Medical Virology vol 22 pp 152–210 Basel: Karger;
    [Google Scholar]
  13. O’Callaghan D. J., Randall C. C., Gentry G. A. 1972a; Herpesvirus replication in vivo . Virology 49:784–793
    [Google Scholar]
  14. O’Callaghan D. J., Rogers J. W., Randall C. C. 1972b; Amino acid composition of equine abortion (herpes) virus. Virology 47:842–844
    [Google Scholar]
  15. Perdue M. L., Cohen J. C., Kemp M. C., Randall C. C., O’Callaghan D. J. 1975; Characterization of three species of nucleocapsids of equine herpesvirus type 1 (EHV-1). Virology 66:187–205
    [Google Scholar]
  16. Perdue M. L., Cohen J. C., Randall C. C., O’Callaghan D. J. 1976; Biochemical studies of the maturation of herpesvirus nucleocapsid species. Virology 74:194–208
    [Google Scholar]
  17. Perdue M. L., Kemp M. C., Randall C. C., O’Callaghan D. J. 1974; Studies of the molecular anatomy of the L-M cell strain of Equine Herpes Virus type 1. Proteins of the nucleocapsids and intact virion. Virology 59:201–216
    [Google Scholar]
  18. Randall C. C., Bracken E. C. 1957; Studies on hepatitis in hamsters infected with equine abortion virus. I. Sequential development of inclusions and the growth cycle. American Journal of Pathology 33:709–727
    [Google Scholar]
  19. Roizman B., Furlong D. 1974; The replication of herpesviruses. In Comprehensive Virology vol 3 pp 229–403
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-3-585
Loading
/content/journal/jgv/10.1099/0022-1317-37-3-585
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error