1887

Abstract

SUMMARY

A temperature-sensitive mutant of is described which produces PBSX phage at a non-permissive temperature (47 °C). The mutant is, in the properties tested, phenotypically identical to the mutant reported by Siegel & Marmur (1969). Its mutation () maps in the same chromosomal region () in which is located; the two mutations are shown to be distinct but probably affect the same function. Double mutants carrying the mutations and (which blocks the induction of PBSX by mitomycin C) do not produce PBSX at a non-permissive temperature but retain their thermosensitivity. mutants display a reduced rate of RNA synthesis at the non-permissive temperature. Such a phenotype is lost in double mutants, revealing that it is associated with PBSX induction. The nature of the primary lesion that leads to PBSX induction in mutants remains to be ascertained.

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1978-04-01
2024-03-29
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References

  1. Buxton R. S. 1976; Prophage mutation causing heat inducibility of defective Bacillus subtilis bacteriophage PBSX. Journal of Virology 20:22–28
    [Google Scholar]
  2. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12. Journal of Bacteriology 60:17–28
    [Google Scholar]
  3. Galizzi A., Siccardi A. G., Mazza G., Canosi U., Polsinelli M. 1976; A recombination test to classify mutants of Bacillus subtilis of identical phenotype. Genetical Research 27:47–58
    [Google Scholar]
  4. Garro A. J., Hammer P., Recht B. 1976; Biochemical and genetic analysis of the defective Bacillus subtilis bacteriophage PBSX. In Microbiology pp 340–349 Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  5. Haas M., Yoshikawa H. 1969a; Defective bacteriophage PBSX in Bacillus subtilis. I. Induction purification and physical properties of bacteriophage and its DNA. Journal of Virology 3:233–247
    [Google Scholar]
  6. Haas M., Yoshikawa H. 1969b; Defective bacteriophage PBSX in B. subtilis. III. Properties of adenine-16 marker in purified bacteriophage DNA. Journal of Virology 4:844–850
    [Google Scholar]
  7. Hoch J. A., Barat M., Anagnostopoulos C. 1967; Transformation and transduction in recombination-defective mutants of Bacillus subtilis. Journal of Bacteriology 93:1925–1937
    [Google Scholar]
  8. Karamata D., Gross J. D. 1970; Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Molecular and General Genetics 108:277–287
    [Google Scholar]
  9. Kirby E. P., Jacob F., Goldthwait D. A. 1967; Prophage induction and filament formation in a mutant strain of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 58:1903–1910
    [Google Scholar]
  10. Lacks S., Hotchkiss R. 1960; A study of the genetic material determining an enzyme activity in Pneumococcus. Biochimica et Biophysica Acta 39:508–517
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  12. Mazza O., Fortunato A., Ferrari E., Canosi U., Falaschi A., Polsinelli M. 1975; Genetic and enzymic studies on the recombination process in Bacillus subtilis. Molecular and General Genetics 136:9–30
    [Google Scholar]
  13. Okamoto K., Mudd J. A., Mangan J., Huang W. M., Subbaiah T. V., Marmur J. 1968a; Properties of the defective phage of Bacillus subtilis. Journal of Molecular Biology 34:413–428
    [Google Scholar]
  14. Okamoto K., Mudd J. A., Marmur J. 1968b; Conversion of Bacillus subtilis DNA to phage DNA following mitomycin C induction. Journal of Molecular Biology 34:429–437
    [Google Scholar]
  15. Seaman E., Tarmy E., Marmur J. 1964; Inducible phages of Bacillus subtilis. Biochemistry 3:607–613
    [Google Scholar]
  16. Schuster H., Beyersmann D., Mikolajczyk M., Schlicht M. 1973; Prophage induction by high temperature in thermosensitive dna mutants lysogenic for bacteriophage lambda. Journal of Virology 11:879–885
    [Google Scholar]
  17. Siccardi A. G., Ferrari F. A., Mazza G., Galizzi A. 1976; Identification of coreplicating chromosomal sectors in Bacillus subtilis by nitrosoguanidine-induced comutation. Journal of Bacteriology 125:755–761
    [Google Scholar]
  18. Siegel E. C., Marmur J. 1969; Temperature-sensitive induction of bacteriophage in Bacillus subtilis 168. Journal of Virology 4:610–618
    [Google Scholar]
  19. Spizizen J. 1958; Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonuclease. Proceedings of the National Academy of Sciences of the United States of America 44:1072–1078
    [Google Scholar]
  20. Stewart C. R. 1969; Physical heterogeneity among Bacillus subtilis deoxyribonucleic acid molecules carrying particular genetics markers. Journal of Bacteriology 98:1239–1247
    [Google Scholar]
  21. Stickler D. J., Tucker R. G., Kay D. 1965; Bacteriophage like particles released from Bacillus subtilis after induction with hydrogen peroxide. Virology 26:142–145
    [Google Scholar]
  22. Subbaiah T. V., Goldthwaite C. D., Marmur J. 1965; Nature of bacteriophages induced in Bacillus subtilis. In Evolving Genes and Proteins pp 435–446 Edited by Bryson V., Vogel H. J. New York: Academic Press Inc;
    [Google Scholar]
  23. Takahashi J. 1963; Transducing phages for Bacillus subtilis. Journal of General Microbiology 31:211–217
    [Google Scholar]
  24. Thurm P., Garro A. J. 1975; Isolation and characterization of prophage mutants of the defective Bacillus subtilis bacteriophage PBSX. Journal of Virology 16:184–191
    [Google Scholar]
  25. Yamagishi H., Takahashi I. 1968; Transducing particles of PBS-l. Virology 36:639–645
    [Google Scholar]
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