1887

Abstract

SUMMARY

The sizes of non-covalently linked RNA subunits isolated from highly leukaemogenic Friend virus derived from the plasma (PV, plasma virus) of leukaemic mice were compared to the RNA subunits isolated from low leukaemogenic Friend virus grown in tissue culture (TCV, tissue culture virus). Histograms derived from electron microscope measurements showed that about one-half of the plasma virus RNA was 1.4 to 2.5 µm in length, corresponding to a mol. wt. range from 1.8 × 10 to 3.2 × 10 and the other half less than 1.4 µm. In contrast, approx. 50% of the TCV RNA was only 0.7 to 1.6 µm in length (mol. wt. 0.9 × 10 to 2.0 × 10) and the remainder less than 0.7 µm in length regardless of whether the virus RNA was isolated from 3, 9, 36 or 72 h cultures. The histograms suggest size classes for both TCV and PV derived RNA subunits. Data obtained from sucrose gradient sedimentation of heat-denatured FLV RNA agreed with the electron microscope length measurements. The smaller sizes of the TCV RNA subunits are hypothetically related to the limited biological activity of Friend leukaemia virus produced from leukaemic cells in culture. Comparable results were obtained using two different RNA extraction procedures. Contamination of TCV nucleic acid preparations by cellular DNA was observed even when the virions were harvested from short term cultures and purified by isopycnic sucrose gradient centrifugation. Consequently, preparations of intact virus were treated with DNase prior to sucrose gradient purification of the virions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-39-3-475
1978-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/39/3/JV0390030475.html?itemId=/content/journal/jgv/10.1099/0022-1317-39-3-475&mimeType=html&fmt=ahah

References

  1. Bader J. P., Steck F. L. 1969; Analysis of the ribonucleic acid of murine leukemia virus. Journal of Virology 4:454–459
    [Google Scholar]
  2. Barski G., Barbieri D., Youn J. K., Hue G. 1973; Low-leukemogenic variants of Rauscher leukemia virus obtained in long-term cultures at supraoptimal temperature. International Journal of Cancer 12:55–65
    [Google Scholar]
  3. Barski G., Youn J. K. 1965; Immunization against Rauscher mouse leukemia with tissue culture material. Science 149:751–752
    [Google Scholar]
  4. Bassin R. H., Tuttle N., Fischinger P. I. 1971; Rapid cell culture assay technique for murine leukaemia viruses. Nature, London 229:564–566
    [Google Scholar]
  5. Cavalieri L. F. 1974; Extent of double strandedness in avian myeloblastosis virus RNA. Journal of Virology 14:1458–1462
    [Google Scholar]
  6. Cox R. A. 1968; The use of guanidinium chloride in the isolation of nucleic acids. Methods in Enzymology 12B:120–129
    [Google Scholar]
  7. Duesberg P. H., Vogt P. K. 1970; Differences between the ribonucleic acids of transforming and nontransforming avian tumor viruses. Proceedings of the National Academy of Sciences of the United States of America 17:1673–1680
    [Google Scholar]
  8. Duesberg P. H., Vogt P. K. 1973; RNA species obtained from clonal lines of avian sarcoma and avian leukosis virus. Virology 54:207–219
    [Google Scholar]
  9. East J. L., Allen P. T., Knesek J. E., Chan J. C., Bowen J. M., Dmochowski L. 1973; Structural rearrangement and subunit composition of RNA from released Soehner-Dmochowski murine sarcoma virions. Journal of Virology 11:709–720
    [Google Scholar]
  10. Evenson D. P. 1977; Electron microscopy of viral nucleic acids. Methods in Virology 6:219–264
    [Google Scholar]
  11. Evenson D. P., Pla D., Beju D., De Harven E. 1975a; A quantitative comparison between in vivo and in vitro derived Friend leukemia virus. International Journal of Cancer 16:819–829
    [Google Scholar]
  12. Evenson D. P., Scotto A., Pavlovec A., Hamilton M., De Harven E. 1975b; Electron microscopy of a tumor virus RNA. Proceedings of the Electron Microscopy Society of America 35:646–647
    [Google Scholar]
  13. Friend C., Patuleia M. C., De Harven E. 1966; Erythrocytic maturation in vitro of murine (Friend) virus-induced leukemic cells. Journal of the National Cancer Institute Monograph 22:505–522
    [Google Scholar]
  14. Granboulan N., Huppert J., Lacour F. 1966; Examen au microscope electronique du RNA du virus de la myeloblastose aviaire. Journal of Molecular Biology 16:571
    [Google Scholar]
  15. Heine U. I., Weber G. H., Cottler-Fox M., Layard M. W., Stephenson M. L., Zamecnik P. C. 1975; Analysis of oncornavirus RNA subunits by electron microscopy. Proceedings of the National Academy of Sciences of the United States of America 72:3716–3720
    [Google Scholar]
  16. Kleinschmidt A. K., Zahn R. K. 1959; Deoxyribonucleic acid molecules in protein-mixed films. Zeit-schrift für Naturforschung 14B:770
    [Google Scholar]
  17. Kolakofsky D., Boy De La Tour E., Delius H. 1974; Molecular weight determination of Sendai and Newcastle disease virus RNA. Journal of Virology 13:261–268
    [Google Scholar]
  18. Leis J. P., Hurwitz J. 1972; RNA-dependent DNA polymerase activity of RNA tumor viruses. II. Directing influence of RNA in the reaction. Journal of Virology 9:130–142
    [Google Scholar]
  19. Loll H., Stutz E. 1968; The use of sodium and lithium dodecyl sulfate in nucleic acid isolation. Methods in Enzymology 12B:129–155
    [Google Scholar]
  20. Maisel J., Klement V., Lai M. M. C., Ostertag W., Duesberg P. 1973; Ribonucleic acid components of murine sarcoma and leukemia viruses. Proceedings of the National Academy of Sciences of the United States of America 70:3536–3540
    [Google Scholar]
  21. Mangel W. F., Delius H., Duesberg P. H. 1974; Structure and molecular weight of the 60-70S RNA and the 30-40S RNA of the Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 71:4541–4545
    [Google Scholar]
  22. Mcclain K., Kirsten W. H. 1972; Electrophoretic analysis of the RNA from a mouse leukemia virus. Cancer Research 32:1470–1475
    [Google Scholar]
  23. Pavlovec A., Evenson D. P., Hamilton M. G. 1978; The use of guanidine-HCl for the isolation of both RNA and protein from RNA tumour viruses. Journal of General Virology 40: (in the press)
    [Google Scholar]
  24. Riggin C. H., Bondurant M. C., Mitchell W. M. 1974; Differences between murine leukemia virus and murine sarcoma virus: effects of virion age and multiplicity of infection on viral RNA. Intervirology 2:209–221
    [Google Scholar]
  25. Robberson D. L., Aloni Y., Attardi G., Davidson N. 1971; Expression of the mitochondrial genome in HeLa cells. VI. Size determination of mitochondrial ribosomal RNA by electron microscopy. Journal of Molecular Biology 60:473–484
    [Google Scholar]
  26. Sato T., Friend C., De Harven E. 1971; Ultrastructural changes in Friend erythroleukemia cells treated with dimethyl sulfoxide. Cancer Research 31:1402–1417
    [Google Scholar]
  27. Scherrer K., Darnell J. E. 1962; Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochemical and Biophysical Research Communications 7:486–490
    [Google Scholar]
  28. Weber G. H., Heine U., Cottler-Fox M., Beaudreau G. S. 1974; Visualization of single-stranded nucleic acid of RNA tumor virus with the electron microscope. Proceedings of the National Academy of Sciences of the United States of America 71:1887–1890
    [Google Scholar]
  29. Zimmerman S. B., Sandeen G. 1966; The ribonuclease activity of crystallized pancreatic deoxyribonuclease. Analytical Biochemistry 14:269–277
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-39-3-475
Loading
/content/journal/jgv/10.1099/0022-1317-39-3-475
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error