f Regulation of the Interferon System: Evidence that Vero Cells have a Genetic Defect in Interferon Production
- Authors: Jean M. Emeny*, Michael J. Morgan†
- First Published Online: 01 April 1979, Journal of General Virology 43: 247-252, doi: 10.1099/0022-1317-43-1-247
- Subject: Articles
- Issue Published:
A clone of Vero cells was isolated and shown to be totally unable to synthesize interferon and insensitive to the toxic effect of poly(rI).poly(rC) treatment. Cells of this clone and mouse L cells were fused by treatment with polyethylene glycol or Sendai virus. Hybrid cell clones were isolated following selection in medium containing hypoxanthine, thymidine and ouabain. The hybrids were sensitive to the antiviral effect of poly(rI).poly(rC) and synthesized mouse, but not primate, interferon. It is proposed that in Vero cells, the gene for interferon synthesis is defective or absent.
Present address: Cancer Biology Research Laboratory, Department of Radiology, Stanford University Medical Center, Stanford, California 94305, U.S.A.
To whom correspondence should be addressed.
© Society for General Microbiology 1979 | Published by the Microbiology Society
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