1887

Abstract

SUMMARY

Macrophages harvested from the peritoneal cavities of mice of several strains were permissive to infection with murine cytomegalovirus (MCMV). Macrophages from six mouse strains released equivalent amounts of plaque-forming virus into the culture fluids and cells from three mouse strains scored similarly in numbers of infectious centres. Twenty to 50% of the infected macrophages obtained after thioglycollate activation formed infectious centres. When studied by hybridization, more than 82% of infected macrophages (with or without thioglycollate activation) contained MCMV DNA.

Macrophages obtained from latently infected mice were examined for their content of MCMV. Using co-cultivation assays, MCMV was frequently recovered from thioglycollate activated macrophages harvested from latently infected mice but only rarely recovered from non-activated macrophages. MCMV DNA-mouse DNA hybridization assays revealed four to seven virus genome DNA copies per 100 cells. These studies indicate that macrophages harvested from mice susceptible (BALB/cSt) or resistant (C3H) to MCMV infection replicated virus equivalently and that macrophages are a reservoir of MCMV during latent and chronic infections. Activation of macrophages may be one of the important steps leading to the exacerbation of latent infections.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-44-2-349
1979-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/44/2/JV0440020349.html?itemId=/content/journal/jgv/10.1099/0022-1317-44-2-349&mimeType=html&fmt=ahah

References

  1. Allison A. C. 1974; On the role of mononuclear phagocytes in immunity against viruses. In Progress in Medical Virology vol 18 pp 15–31 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
  2. Brautigam A. R., Oldstone M. B. A. 1978; Detection of cytomegalovirus within mouse reproductive tissues during latent and acute infection. Fourth International Congress for Virology The Hague, The Netherlands: (abstract)
    [Google Scholar]
  3. Britten R. J., Kohne D. E. 1968; Repeated sequences in DNA. Science 161:529–540
    [Google Scholar]
  4. Brodsky I., Rowe W. P. 1958; Chronic subclinical infection with mouse salivary gland virus. Proceedings of the Society for Experimental Biology and Medicine 99:654–655
    [Google Scholar]
  5. Caul E. O., Clarke S. K. R., Mott M. G., Perham T. G. M., Wilson R. S. E. 1971; Cytomegalovirus infections after open heart surgery. Lancet i:777–781
    [Google Scholar]
  6. Chalmers J. E., Mackenzie J. S., Stanley N. F. 1977; Resistance to murine cytomegalovirus linked to the major histocompatibility complex of the mouse. Journal of General Virology 37:107–114
    [Google Scholar]
  7. Craighead J. E., Hanshaw J. B., Carpenter C. B. 1967; Cytomegalovirus infection after renal allotransplantation. Journal of the American Medical Association 201:725–728
    [Google Scholar]
  8. Dutko F. J., Oldstone M. B. A. 1978; Murine cytomegalovirus infects spermatogenic cells. Proceedings of the National Academy of Sciences of the United States of America (in the press)
    [Google Scholar]
  9. Gonzalez-Serva A., Hsiung G. D. 1978; Expression of herpesvirus in adherent cells derived from bone marrow of latently infected guinea pigs. American Journal of Pathology 91:483–496
    [Google Scholar]
  10. Henson D., Smith R. D., Gehrke J. 1966; Non-fatal mouse cytomegalovirus hepatitis. American Journal of Pathology 49:871–888
    [Google Scholar]
  11. Hirsch M. S., Zisman B., Allison A. C. 1970; Macrophages and age-dependent resistance to herpes simplex virus in mice. Journal of Immunology 104:1160–1165
    [Google Scholar]
  12. Huang E. S., Chen S., Pagano J. S. 1973; Human cytomegalovirus. I. Purification and characterization of viral DNA. Journal of Virology 12:1473–1481
    [Google Scholar]
  13. Johnson R. T. 1964; The pathogenesis of herpes virus encephalitis. II. A cellular basis for the development of resistance with age. Journal of Experimental Medicine 120:359–374
    [Google Scholar]
  14. Kingsbury D. T., Lerner R. A. 1974; Encapsulation of lymphocyte DNA by vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 71:1753–1757
    [Google Scholar]
  15. Kohne D. E. 1968; Isolation and characterization of bacterial ribosomal RNA cistrons. Biophysical Journal 8:1104–1118
    [Google Scholar]
  16. Lang D. J., Scolnick E. M., Willerson J. T. 1968; Association of cytomegalovirus infection with the post perfusion syndrome. New England Journal of Medicine 278:1147–1149
    [Google Scholar]
  17. Mannini A., Medearis D. N. 1961; Mouse salivary gland virus infections. American Journal of Hygiene 73:329–343
    [Google Scholar]
  18. Medearis D. N. 1964; Mouse cytomegalovirus infection. II. Observations during prolonged infection. American Journal of Hygiene 80:103–112
    [Google Scholar]
  19. Mims C. A. 1964; Aspects of the pathogenesis of virus diseases. Bacteriological Reviews 28:30–71
    [Google Scholar]
  20. Mims C. A., Gould J. 1978; The role of macrophages in mice infected with murine cytomegalovirus. Journal of General Virology 41:143–153
    [Google Scholar]
  21. Olding L. B., Jensen F. C., Oldstone M. B. A. 1975; Pathogenesis of cytomegalovirus infection. I. Activation of virus from bone marrow derived lymphocytes by in vitro allogenic reaction. Journal of Experimental Medicine 141:561–572
    [Google Scholar]
  22. Olding L. B., Kingsbury D. T., Oldstone M. B. A. 1976; Pathogenesis of cytomegalovirus infection. Distribution of viral products, immune complexes and autoimmunity during latent murine infection. Journal of General Virology 33:267–280
    [Google Scholar]
  23. Oldstone M. B. A., Haspel M. V., Pellegrino M. A., Kingsbury D. T., Olding L. B. 1976; Histocompatibility complex and virus infection, latency and activation. Transplantation Reviews 31:225–239
    [Google Scholar]
  24. Reynolds D. W., Stagno S., Hosty T. S., Tiller M., Alford C. A. Jun 1973; Maternal cytomegalovirus excretion and perinatal infection. New England Journal of Medicine 289:1–5
    [Google Scholar]
  25. Reynolds D. W., Stagno S., Stubbs K. G., Dahle A. J., Livingston M. M., Saxon S. S., Alford C. A. 1974; Inapparent congenital cytomegalovirus infection with elevated cord IgM levels. Causal relation with auditory and mental deficiency. New England Journal of Medicine 290:291–296
    [Google Scholar]
  26. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  27. Selgrade M. K., Osborn J. E. 1974; Role of macrophages in resistance to murine cytomegalovirus. Infection and Immunity 10:1383–1390
    [Google Scholar]
  28. Silverstein S. C. 1975 In Mononuclear Phagocytes in Immunity, Infection and Pathology chapter 36, pp 557–573 Edited by Van Furth R. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  29. Tegtmeyer P. J., Craighead J. E. 1968; Infection of adult mouse macrophages in vitro with cytomegalovirus. Proceedings of the Society for Experimental Biology and Medicine 129:690–694
    [Google Scholar]
  30. Unanue E. R. 1976; Secretory function of mononuclear phagocytes. American Journal of Pathology 83:396–417
    [Google Scholar]
  31. Weller T. H. 1971a; The cytomegaloviruses: ubiquitous agents with protein clinical manifestations (part one). New England Journal of Medicine 285:203–214
    [Google Scholar]
  32. Weller T. H. 1971b; The cytomegaloviruses: ubiquitous agents with protein clinical manifestations (part two). New England Journal of Medicine 285:267–274
    [Google Scholar]
  33. Zisman B., Hirsch M. S., Allison A. C. 1970; Selective effects of anti-macrophage serum, silica and antilymphocyte serum on pathogenesis of herpes virus infection of young adult mice. Journal oj Immunology 104:1155–1159
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-2-349
Loading
/content/journal/jgv/10.1099/0022-1317-44-2-349
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error