%0 Journal Article %A Whalley, J. Millar %A Robertson, Graham R. %A Davison, Andrew J. %T Analysis of the Genome of Equine Herpesvirus Type 1: Arrangement of Cleavage Sites for Restriction Endonucleases EcoRI, BglII and BamHI %D 1981 %J Journal of General Virology, %V 57 %N 2 %P 307-323 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-57-2-307 %K restriction endonuclease maps %K EHV-1 %K genome arrangement %I Microbiology Society, %X SUMMARY The genome of an Australian isolate of equine herpesvirus type 1 (equine abortion virus) has been analysed using the restriction endonucleases EcoRI, BglII and BamHI, and a physical map constructed. Terminal fragments were identified by exonuclease treatments, and linkage of fragments was deduced by a combination of single- and double-digest experiments and cross-blot hybridizations. The genome has a mol. wt. of 100 × 106 and is comprised of a short unique region bounded by repetitive sequences, which is present in both orientations in approximately equal amounts in the DNA population, and a long unique region existing in only one orientation. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-57-2-307