f Isolation and Properties of a Pseudomonas acidovorans Bacteriophage
- Authors: A. M. B. Kropinski*, R. A. J. Warren
- J. Gen. Virol., January 1970 6: 85-93, doi: 10.1099/0022-1317-6-1-85
- Subject: Articles
- Published Online:
The lytic bacteriophage φW-14 was isolated from sewage using Pseudomonas acidovorans no. 14 as host. The phage had an icosahedral head some 85 nm. in diameter and a contractile tail some 140 nm. long. φW-14 formed plaques on only a few strains of P. acidovorans. The phage gave biphasic absorption kinetics, with an adsorption constant of 1.9 × 10-9 ml./min. The latent period was 60 min. and the burst size was about 300. The burst size was dependent upon culture age. The k u.v. for inactivation of φW-14 was 4.35 min.-1. P. acidovorans was shown to possess a potent photoreactivating system. The heat of inactivation of φW-14 was calculated to be 75,700 cal./mole. The phage gave biphasic thermal inactivation kinetics at 55° and 60° but not at 65°. The phage mutated spontaneously to a different plaque type. This mutation affected the adsorptive properties, the thermal sensitivity and the burst size of the phage.
Present Address: Department of Biology, University of Calgary, Calgary, Alberta, Canada.
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