1887

Abstract

SUMMARY

A panel of 125 monoclonal antibodies (IgG) was raised against the haemagglutinin of an early representative of the Hong Kong (H3N2) subtype of influenza. They were classified into groups based on their cross-reactions with 16 other virus strains from the same subtype. This classification was performed using methods of numerical taxonomy. Statistical tests supported the validity of the grouping. Ten such groups were identified. Nine antibodies remained unclassified. The locations on the haemagglutinin molecule of amino acid residues influencing the binding of each antibody group were estimated. This was achieved by a study of antibody cross-reactive profiles, coupled with previously published locations of amino acid changes in the primary sequence of different haemagglutinins, and their positions in the tertiary structure of the molecule. The locations of the amino acids affecting antibody binding overlapped between the different antibody groups forming a continuous ring surrounding the probable cell-receptor pocket. The amino acids affecting the binding of each antibody group may or may not represent the actual antibody binding sites. The importance of the different sites of amino acid variation in the haemagglutinin during evolution of the virus is discussed.

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1982-09-01
2024-04-19
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References

  1. Amzel L. M., Poljak R. J. 1979; Three-dimensional structure of immunoglobulins. Annual Review of Biochemistry 48:961–997
    [Google Scholar]
  2. Both G. W., Sleigh M. J. 1980; Complete nucleotide sequence of the haemagglutinin gene from a human influenza virus of the Hong Kong subtype. Nucleic Acids Research 8:2561–2575
    [Google Scholar]
  3. Both G. W., Sleigh M. J. 1981; Conservation and variation in the hemagglutinins of Hong Kong subtype influenza viruses during antigenic drift. Journal of Virology 39:663–672
    [Google Scholar]
  4. Both G. W., Sleigh M. J., Bender V. J., Moss B. A. 1980 A comparison of antigenic variation in Hong Kong influenza virus haemagglutinins at the nucleic acid level. In Structure and Variation in Influenza Virus pp. 81–89 Edited by Laver W. G., Air G. M. New York: Elsevier;
    [Google Scholar]
  5. Brand C. M., Skehel J. J. 1972; Crystalline antigen from the influenza virus envelope. Nature, London 238:145–147
    [Google Scholar]
  6. Breschkin A. M., Ahern J., White D. O. 1981; Antigenic determinants of influenza virus haemagglutinin. VIII. Topography of the antigenic regions of influenza virus haemagglutinin determined by competitive radio immunoassay with monoclonal antibodies. Virology 113:130–140
    [Google Scholar]
  7. East I. J., Todd P. E., Leach S. J. 1980; On topographic antigenic determinants in myoglobins. Molecular Immunology 17:519–525
    [Google Scholar]
  8. Eckert E. A. 1973; Properties of an antigenic glycoprotein isolated from influenza virus haemagglutinin. Journal of Virology 11:183–192
    [Google Scholar]
  9. Fazekas de St., Groth S., Cairns H. J. F. 1952; Influenza virus multiplication. IV. Definition of constants and general discussion. Journal of Immunology 69:173–181
    [Google Scholar]
  10. Fazekas de St., Groth S., Graham D. M. 1954; The production of incomplete virus particles among influenza strains: experiments in eggs. British Journal of Experimental Pathology 35:60–74
    [Google Scholar]
  11. Fazekas de St., Groth S., Hannoun C. 1973; Selection par pression immunologique de mutants dominants du virus de la grippe A (Hong Kong). Comptes rendus hebdomadaires des séances de l'Académie des sciences, serie D 276:1917–1920
    [Google Scholar]
  12. Fazekas de St., Groth S., Withell J., Lafferty K. J. 1958; An improved assay method for neutralising antibodies against influenza viruses. Journal of Hygiene 56:415–426
    [Google Scholar]
  13. Galfre G., Howe S. C., Milstein C. 1977; Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature, London 266:550–552
    [Google Scholar]
  14. Gerhard W., Yewdell J., Frankel M. E., Webster R. 1981; Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies. Nature, London 290:713–717
    [Google Scholar]
  15. Kohler G., Milstein C. 1976; Derivation of specific antibody-producing tissue culture and tumour lines by cell fusion. European Journal of Immunology 6:511–519
    [Google Scholar]
  16. Laver W. G., Air G. M., Dopheide T. A., Ward C. W. 1980; Amino acid sequence changes in the haemagglutinin of A/Hong Kong (H3N2) influenza virus during the period. 1968–77 Nature, London 283:454–457
    [Google Scholar]
  17. Lubeck M. D., Gerhard W. 1981; Topological mapping of antigenic sites on the influenza A/PR/8/34 virus hemagglutinin using monoclonal antibodies. Virology 113:64–72
    [Google Scholar]
  18. Min jou W., Verhoeyen M., Devos R., Saman E., Fang R., Huylebrook D., Fiers W. 1980; Complete structure of the haemagglutinin gene from the human influenza A/Victoria/3/75 (H3N2) strain as determined from cloned DNA. Cell 19:683–696
    [Google Scholar]
  19. Moss B. A., Underwood P. A., Bender V. J., Whittaker R. G. 1980 Antigenic drift in the haemagglutinin from various strains of influenza virus A/Hong Kong/68 (H3N2). In Structure and Variation in Influenza Virus pp. 329–338 Edited by Laver W. G., Air G. M. New York: Elsevier;
    [Google Scholar]
  20. Shulman M., Wilde C. D., Kohler G. 1978; A better cell line for making hybridomas secreting specific antibodies. Nature, London 276:269–270
    [Google Scholar]
  21. Sleigh M. J., Both G. W., Underwood P. A., Bender V. J. 1981; Antigenic drift in the haemagglutinin of the Hong Kong influenza subtype: correlation of amino acid changes with alterations in viral antigenicity. Journal of Virology 37:845–853
    [Google Scholar]
  22. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy San Francisco: W. H. FreemanCo;
    [Google Scholar]
  23. Sutcliffe J. G., Shinnick T. M., Green N., Liu F. T., Niman H. I., Lerner R. A. 1980; Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product. Nature, London 287:801–805
    [Google Scholar]
  24. Verhoeyen M., Fang R., Min jou W., Devos R., Huylebroeck D., Samas E., Fiers W. 1980; Antigenic drift between the haemagglutinin of the Hong Kong influenza strains A/Aichi/2/68 and A/Victoria/3/75. Nature, London 286:771–776
    [Google Scholar]
  25. Ward C. W., Dopheide T. A. A. 1980 The Hong Kong (H3) haemagglutinin. Complete amino acid sequence and oligosaccharide distribution for the heavy chain of A/Memphis/102/72. In Structure and Variation in Influenza Virus pp. 27–37 Edited by Laver W. G., Air G. M. New York: Elsevier;
    [Google Scholar]
  26. Webster R. G., Laver W. G. 1980 Antigenic drift in Hong Kong (H3N2) influenza viruses: selection of variants with potential epidemiological significance using monoclonal antibodies. In Structure and Variation in Influenza Virus pp. 283–293 Edited by Laver W. G., Air G. M. New York: Elsevier;
    [Google Scholar]
  27. Wiley D. C., Wilson I. A., Skehel J. J. 1981; Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature, London 289:373–378
    [Google Scholar]
  28. Wilson I. A., Skehel J. J., Wiley D. C. 1981; Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 À resolution. Nature, London 289:366–373
    [Google Scholar]
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