%0 Journal Article %A Marsden, H. S. %A Lang, J. %A Davison, A. J. %A Hope, R. G. %A Macdonald, D. M. %T Genomic Location and Lack of Phosphorylation of the HSV Immediate-Early Polypeptide IE 12 %D 1982 %J Journal of General Virology, %V 62 %N 1 %P 17-27 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-62-1-17 %K phosphoproteins %K HSV %K gene mapping %K immediate-early proteins %I Microbiology Society, %X SUMMARY Herpes simplex virus type 1 (HSV-1) induces an immediate-early (IE) polypeptide IE 12. An equivalent polypeptide coded by HSV-2 which migrated slightly more slowly on SDS-polyacrylamide gels was identified and designated IE 12.3. Analysis of the serotype of the IE polypeptide induced by five HSV-1/HSV-2 intertypic recombinants when correlated with their genome structures showed that IE 12/IE 12.3 mapped in the region spanning the TRS/US junction. Unlike four other IE polypeptides induced by HSV-1 (IEs 175, 110, 68 and 63), IE 12 was not detectably phosphorylated. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-62-1-17