Genomic Location and Lack of Phosphorylation of the HSV Immediate-Early Polypeptide IE 12 Marsden, H. S. and Lang, J. and Davison, A. J. and Hope, R. G. and Macdonald, D. M.,, 62, 17-27 (1982), doi = https://doi.org/10.1099/0022-1317-62-1-17, publicationName = Microbiology Society, issn = 0022-1317, abstract= SUMMARY Herpes simplex virus type 1 (HSV-1) induces an immediate-early (IE) polypeptide IE 12. An equivalent polypeptide coded by HSV-2 which migrated slightly more slowly on SDS-polyacrylamide gels was identified and designated IE 12.3. Analysis of the serotype of the IE polypeptide induced by five HSV-1/HSV-2 intertypic recombinants when correlated with their genome structures showed that IE 12/IE 12.3 mapped in the region spanning the TRS/US junction. Unlike four other IE polypeptides induced by HSV-1 (IEs 175, 110, 68 and 63), IE 12 was not detectably phosphorylated., language=, type=