%0 Journal Article %A Walker, J. R. %A Nagington, J. %A Scott, G. M. %A Secher, D. S. %T An Immunoradiometric Assay of Serum Interferon using a Monoclonal Antibody %D 1982 %J Journal of General Virology, %V 62 %N 1 %P 181-185 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-62-1-181 %K monoclonal antibody %K IFN-α %K immunoradiometric assay %K human serum %I Microbiology Society, %X SUMMARY An immunoradiometric assay for human interferon-α (HuIFN-α) has been adapted for the assay of low concentrations of HuIFN-α in human serum. The sensitivity of the assay is 5 to 10 IU/ml and the coefficient of variation less than 10%. The assay was shown to compare well with a biological (antiviral) assay in the measurement of serum interferon following intramuscular injection of HuIFN-α in nine volunteers. Serum interferon was also measured in the serum from 250 normal human donors. Two donors appeared to have detectable levels of HuIFN-α. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-62-1-181