An Immunoradiometric Assay of Serum Interferon using a Monoclonal Antibody Walker, J. R. and Nagington, J. and Scott, G. M. and Secher, D. S.,, 62, 181-185 (1982), doi = https://doi.org/10.1099/0022-1317-62-1-181, publicationName = Microbiology Society, issn = 0022-1317, abstract= SUMMARY An immunoradiometric assay for human interferon-α (HuIFN-α) has been adapted for the assay of low concentrations of HuIFN-α in human serum. The sensitivity of the assay is 5 to 10 IU/ml and the coefficient of variation less than 10%. The assay was shown to compare well with a biological (antiviral) assay in the measurement of serum interferon following intramuscular injection of HuIFN-α in nine volunteers. Serum interferon was also measured in the serum from 250 normal human donors. Two donors appeared to have detectable levels of HuIFN-α., language=, type=