@article{mbs:/content/journal/jgv/10.1099/0022-1317-64-1-1, author = "Davison, A. J. and Wilkie, N. M.", title = "Inversion of the Two Segments of the Herpes Simplex Virus Genome in Intertypic Recombinants", journal= "Journal of General Virology", year = "1983", volume = "64", number = "1", pages = "1-18", doi = "https://doi.org/10.1099/0022-1317-64-1-1", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-1-1", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "inversion", keywords = "a sequence", keywords = "HSV", keywords = "recombinants", abstract = "SUMMARY We have analysed by restriction site mapping the structures of the termini and L-S joint in several HSV-1/HSV-2 intertypic recombinants, including Bx1(28-1), the virion DNA of which has a marked overabundance of one orientation of the L segment, and subclones of Bx1(28-1). All recombinants with both orientations of L present in equal amounts contain TRL and IRL regions derived at least in part from the same parent (HSV-1 or HSV-2) as a result of previously undetected crossovers in these regions. Recombinants with a predominance of one orientation of L have TRL and IRL regions derived from different parents. Homology between a sequences alone at the L terminus and L-S joint is sufficient for normal inversion of L. Analysis of another recombinant, RE4, which fails to invert normally in both L and S, suggests that normal inversion of S is dependent upon the presence of TRS and IRS regions derived at least in part from the same parent. We conclude that segment inversion specifically depends upon the a sequence, that the process of DNA replication and maturation does not necessarily produce molecules with identical a sequences, and that direct ligation of termini may occur during DNA replication.", }