RT Journal Article SR Electronic(1) A1 Davison, A. J. A1 Wilkie, N. M.YR 1983 T1 Inversion of the Two Segments of the Herpes Simplex Virus Genome in Intertypic Recombinants JF Journal of General Virology, VO 64 IS 1 SP 1 OP 18 DO https://doi.org/10.1099/0022-1317-64-1-1 PB Microbiology Society, SN 1465-2099, AB SUMMARY We have analysed by restriction site mapping the structures of the termini and L-S joint in several HSV-1/HSV-2 intertypic recombinants, including Bx1(28-1), the virion DNA of which has a marked overabundance of one orientation of the L segment, and subclones of Bx1(28-1). All recombinants with both orientations of L present in equal amounts contain TRL and IRL regions derived at least in part from the same parent (HSV-1 or HSV-2) as a result of previously undetected crossovers in these regions. Recombinants with a predominance of one orientation of L have TRL and IRL regions derived from different parents. Homology between a sequences alone at the L terminus and L-S joint is sufficient for normal inversion of L. Analysis of another recombinant, RE4, which fails to invert normally in both L and S, suggests that normal inversion of S is dependent upon the presence of TRS and IRS regions derived at least in part from the same parent. We conclude that segment inversion specifically depends upon the a sequence, that the process of DNA replication and maturation does not necessarily produce molecules with identical a sequences, and that direct ligation of termini may occur during DNA replication., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-1-1