1887

Abstract

SUMMARY

DNA polymerase activity present in multiple nucleocapsid nuclear polyhedrosis virus-infected cells has been analysed by chromatography on DNA-cellulose and phosphocellulose columns. In infected cells a new fraction of activity was found to bind to the columns more strongly that did polymerase activity in uninfected cells. The infected-cell-specific DNA polymerase was purified by a combination of DNA-cellulose chromatography of crude extracts and phospho-cellulose chromatography of the semi-purified activity. The final product contained a single polypeptide of molecular weight 126 000 which was not found in uninfected cells. The purified enzyme was inhibited by aphidicolin and []-5-(2-bromovinyl)-2′-deoxyuridine triphosphate, but not by bromovinyldeoxyuridine. The enzyme was shown to be an early enzyme, probably a delayed early protein, since it was present in cells inhibited by aphidicolin which were locked into the synthesis of early proteins by the drug.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-10-2229
1983-10-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/10/JV0640102229.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-10-2229&mimeType=html&fmt=ahah

References

  1. Allen G. P., O’Callaghan D. J., Randall C. C. 1977; Purification and characterisation of equine herpes virus induced DNA polymerase. Virology 76:395–108
    [Google Scholar]
  2. Challberg M. D., Englund P. T. 1979; Purification and properties of the deoxyribonucleic acid polymerase induced by vaccinia virus. Journal of Biological Chemistry 254:7812–7819
    [Google Scholar]
  3. De Clercq E., Descamps J., Maudgal P. C., Missotten L., Leyten R., Verhelst G., Jones A. S., Walker R. T., Busson R., Vanderhaeghe H., De Somer P. 1980; Selective anti-herpes activity of 5-(2-halogenovinyl)-2′-deoxyuridines and -2′-deoxycytidines. In Developments in Antiviral Therapy pp 21–42 Edited by Collier L. H., Oxford J. London: Academic Press;
    [Google Scholar]
  4. Elliott R. M., Lescott T., Kelly D. C. 1977; Serological relationships of an iridescent virus (type 25) recently isolated from Tipula sp. with two other iridescent viruses (types 2 and 22). Virology 25:336–350
    [Google Scholar]
  5. Huberman J. A. 1981; New views of the biochemistry of eukaryotic DNA replication revealed by aphidicolin, an unusual inhibitor of DNA polymerase a. Cell 23:647–648
    [Google Scholar]
  6. Kelly D. C. 1981; Baculovirus replication: stimulation of thymidine kinase and DNA polymerase activities in Spodoptera frugiperda cells infected with Trichoplusia ni nuclear polyhedrosis virus. Journal of General Virology 52:313–319
    [Google Scholar]
  7. Kelly D. C. 1982; Baculovirus replication. Journal of General Virology 63:1–13
    [Google Scholar]
  8. Kelly D. C., Lescott T. 1981; Baculovirus replication: protein synthesis in Spodoptera frugiperda cells infected with Trichoplusia ni nuclear polyhedrosis virus. Microbiologica 4:35–57
    [Google Scholar]
  9. Kelly D. C., Wang X. 1981; The infectivityof nuclear polyhedrosis virus DNA. Annalesde Virologie 132E:247–259
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Miller L. K., Jewell J. E., Browne D. 1981; Baculovirus induction of a DNA polymerase. Journal of Virology 40:305–308
    [Google Scholar]
  12. Pedrali-Noy G. A., Spadari S. 1980; Mechanism of inhibition of herpes simplex virus and vaccinia virus DNA polymerases by aphidicolin. Journal of Virology 36:457–464
    [Google Scholar]
  13. Powell K. L., Purifoy D. J. M. 1977; Non structural proteins of herpes simplex virus. I. Purification of the induced DNA polymerase. Journal of Virology 24:618–626
    [Google Scholar]
  14. Spadari S., Sala F., Pedrali-Noy G. 1982; Aphidicolin: a specific inhibitor of nuclear DNA replication in eukaryotes. Trends in Biochemical Sciences 7:29–32
    [Google Scholar]
  15. Wang X., Lescott T., De Clercq E., Kelly D. C. 1983; Baculovirus replication: inhibition of Trichoplusia ni multiple nuclear polyhedrosis virus by [E]-5-(2-bromovinvl)-2′-deoxyuridine. Journal of General Virology 64:1221–1227
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-10-2229
Loading
/content/journal/jgv/10.1099/0022-1317-64-10-2229
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error