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Volume 64, Issue 12

Synthesis of Full-length, Virus Genomic DNA by Nuclei of Vaccinia-infected HeLa Cells Free

Abstract

SUMMARY

It is well established that vaccinia virus infection induces the synthesis of virus-specific DNA in cytoplasmic ‘factories’, which are sites of virus-specific transcription. The present study demonstrates that vaccinia virus-specific DNA is synthesized also in the nuclei of infected cells with a similar time course. Direct observation and radiolabelling confirm the integrity of isolated nuclei. Reconstitution experiments and inhibitor studies demonstrate that virus-induced DNA is synthesized within nuclei and does not result from cytoplasmic contamination. Cell-specific DNA synthesis is inhibited completely after infection and nuclei of infected cells then synthesize DNA which co-sediments with virus genomic DNA in denaturing gradients. Restriction endonuclease cleavage and hybridization with a virus-specific probe indicate that this is full-length, virus genomic DNA. The biological implications of this are discussed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): DNA synthesis , nuclei and vaccinia virus
© Journal of General Virology 1983
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1983-12-01
2024-04-16
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Synthesis of Full-length, Virus Genomic DNA by Nuclei of Vaccinia-infected HeLa Cells
J. Gen. Virol. 64, 2561 (1983); https://doi.org/10.1099/0022-1317-64-12-2561
/content/journal/jgv/10.1099/0022-1317-64-12-2561
/content/journal/jgv/10.1099/0022-1317-64-12-2561
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