1887

Abstract

SUMMARY

Infected cell-specific polypeptides (ICSP) in nuclear polyhedrosis virus (MNPV)-infected cells could be radiolabelled with various sugar precursors including mannose, -acetyl glucosamine, -acetyl mannosamine and glucose. Glycosylation occurred mainly late in infection. Eleven polypeptides were glycosylated including the major envelope protein, the polyhedron protein (very late in infection) and a major low molecular weight non-structural polypeptide (also very late in infection). Tunicamycin inhibited MNPV replication and prevented the addition of -acetyl glucosamine and, to a lesser extent, mannose to the appropriate proteins. This had the effect of enhancing the migration in SDS-polyacrylamide gels of some glycopolypeptides but not that of the polyhedron protein. Tunicamycin prevented the envelopment of nucleocapsids both within the nucleus and at the plasma membrane. Polyhedron formation did, however, occur in the presence of tunicamycin.

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1983-09-01
2024-04-19
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References

  1. Adams J. R., Goodwin R. H., Wilcox T. H. 1977; Electron microscopic investigations on invasion and replication of insect baculovirus in vivo and in vitro. Biologic Cellulaire 28:261–268
    [Google Scholar]
  2. Burley S. K., Miller A., Harrap K. A., Kelly D. C. 1982; Structure of the baculovirus nucleocapsid. Virology 120:433–440
    [Google Scholar]
  3. Crumpacker C. S. 1980; Viral glycoproteins in infectious disease processes. Reviews of Infectious Diseases 2:78–103
    [Google Scholar]
  4. Dobos P., Cochran M. A. 1980; Protein synthesis in cells infected with Autographa californica nuclear polyhedrosis virus: the effect of cytosine arabinoside. Virology 103:446–464
    [Google Scholar]
  5. Elliott R. M., Lescott T., Kelly D. C. 1977; Serological relationships of an iridescent virus (type 25) recently isolated from Tipula sp. with two other iridescent viruses (types 2 and 22). Virology 81:309–316
    [Google Scholar]
  6. Gardiner G. R., Stockdale H. 1975; Two tissue culture media for production of lepidopteran cells lines and nuclear polyhedrosis viruses. Journal of Invertebrate Pathology 25:336–340
    [Google Scholar]
  7. Goldstein N. I., McIntosh A. H. 1980; Glycoproteins of nuclear polyhedrosis viruses. Archives of Virology 64:119–126
    [Google Scholar]
  8. Harrap K. A. 1972; The structure of nuclear polyhedrosis viruses. II. The virus particle. Virology 50:124–132
    [Google Scholar]
  9. Hemming F. W. 1982; Control and manipulation of the phosphodolichol pathway of protein N-glycosylation. Bioscience Reports 2:203–221
    [Google Scholar]
  10. Kelly D. C. 1981; Baculovirus replication: electron microscopy of the sequence of infection of Trichoplusia ni nuclear polyhedrosis virus in Spodoptera frugiperda cells. Journal of General Virology 52:313–319
    [Google Scholar]
  11. Kelly D. C. 1982; Baculovirus replication. Journal of General Virology 63:1–13
    [Google Scholar]
  12. Kelly D. C., Lescott T. 1981; Baculovirus replication: protein synthesis in Sphodoptera frugiperda cells infected with Trichoplusia ni nuclear polyhedrosis virus. Microbiologica (Bologna) 4:35–57
    [Google Scholar]
  13. Kelly D. C., Wang X. 1981; The infectivity of nuclear polyhedrosis virus DNA. Annalesde Virologie 133E:247–259
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Schwarz R. T., Datema R. 1980; Inhibitors of protein glycosylation. Trends in Biochemical Sciences 18:65–67
    [Google Scholar]
  16. Stoltz D. B., Pavan C., Da Cunha A. B. 1973; Nuclear polyhedrosis virus: a possible example of de novo intranuclear membrane morphogenesis. Journal of General Virology 19:145–150
    [Google Scholar]
  17. Summers M. D., Volkman L. E. 1976; Comparison of biophysical and morphological properties of occluded and extracellular non-occluded baculovirus from in vivo and in vitro host systems. Journal of Virology 17:962–972
    [Google Scholar]
  18. Takatsuki A., Arima K., Tamura G. 1971; Tunicamycin, a new antibiotic. I. Isolation and characterisation of tunicamycin. Journal of Antibiotics 24:224–231
    [Google Scholar]
  19. Takatsuki A., Knono K., Tamura G. 1975; Inhibition of biosynthesis of polyisoprenol sugars in chick embryo microsomes by tunicamycin. Agricultural and Biological Chemistry 39:2089–2091
    [Google Scholar]
  20. Volkman L. E., Summers M. D., Hsieh C. H. 1976; Occluded and non-occluded nuclear polyhedrosis virus grown in Trichoplusia ni: comparative neutralisation, infectivity and in vitro growth studies. Journal of Virology 19:820–832
    [Google Scholar]
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