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Volume 64, Issue 9

Processing of Glycoproteins Induced by Herpes Simplex Virus Type 1: Sulphation and Nature of the Oligosaccharide Linkages Free

Abstract

SUMMARY

Using the drug tunicamycin we have investigated the nature of the oligosaccharides on herpes simplex virus type 1 (HSV-1)-induced glycoproteins E and Y (gY is a newly identified glycoprotein which has the same apparent mol. wt. as gC but a more basic isoelectric point). Synthesis of both glycoproteins was inhibited by the drug, suggesting they contain -linked oligosaccharides. Our finding, combined with the previous results of other workers, suggests that all the major HSV-induced glycoproteins have this type of carbohydrate modification. All of the major HSV-1-induced glycoproteins are modified by addition of inorganic sulphate. This modification occurs late in their maturation. Most inorganic sulphate appears to be attached to -linked oligosaccharides but some is attached to other parts of glycoprotein E. Using HSV-1/HSV-2 intertypic recombinants, the mapping limits of that part of the glycoprotein E gene coding for differences in mobility between the two serotypes have been further narrowed and are located between coordinates 0.886 and 0.935.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): glycoproteins , HSV-1 , oligosaccharides and processing
© Journal of General Virology 1983
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1983-09-01
2024-04-23
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Processing of Glycoproteins Induced by Herpes Simplex Virus Type 1: Sulphation and Nature of the Oligosaccharide Linkages
J. Gen. Virol. 64, 1943 (1983); https://doi.org/10.1099/0022-1317-64-9-1943
/content/journal/jgv/10.1099/0022-1317-64-9-1943
/content/journal/jgv/10.1099/0022-1317-64-9-1943
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