1887

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Volume 65, Issue 7

Antigenic and Pathogenic Relationships of Three Bovine Rotaviruses and a Porcine Rotavirus Free

Abstract

SUMMARY

The antigenic relationships between three bovine rotaviruses, UK, CP-1 and PP-1, and a porcine rotavirus were investigated; their pathogenicity for piglets was also assessed. After propagation and cloning in cell culture, the four viruses were used to produce convalescent and hyperimmune antisera in gnotobiotic animals. For the UK and PP-1 viruses, reciprocal virus neutralization tests gave ratios of homologous to heterologous titres in the range 57 to >2300. Taking a 20-fold difference in titre to be the criterion of heterogeneity, these two viruses could be regarded as separate serotypes. The third bovine virus, CP-1, was identical to PP-1 in reciprocal neutralization tests but gave a one-way cross-reaction with UK convalescent sera, i.e. it appeared to be intermediate between the two serotypes. UK and CP-1 differed antigenically from the porcine virus as shown by homologous:heterologous neutralization titre ratios of 30 to 12600. The bovine virus, PP-1, however, had a closer relationship with the porcine virus, the homologous:heterologous ratios ranging from 4 to 110. Oral inoculation of piglets with the bovine viruses revealed differences in their pathogenicity. Isolates UK and CP-1 caused no clinical disease and could not be passaged. However, on the first and second passages in pigs, the bovine virus PP-1 and the porcine virus produced clinical disease, excretion of virus in the faeces and seroconversion. The results suggest that PP-1 may be a natural hybrid between a bovine and a porcine rotavirus.

  • Received:
  • Accepted:
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Keyword(s): antigenicity , bovine and porcine , pathogenicity and rotaviruses
© Journal of General Virology 1984
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1984-07-01
2024-04-20
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References

  1. Bartz C. R., Conklin R. H., Tunstall C. B., Steele J. H. 1980; Prevention of murine rotavirus infection with chicken egg yolk immunoglobulin. Journal of Infectious Diseases 142:439–441
     [Google Scholar]
  2. Bastardo J. W., McKimm-Breschkin J. L., Sonza S., Mercer L. D., Holmes I. H. 1981; Preparation and characterization of antisera to electrophoretically purified SA11 virus polypeptides. Infection and Immunity 34:641–647
     [Google Scholar]
  3. Beards G. M., Pilford J. N., Thouless M. E., Flewett T. H. 1980; Rotavirus serotypes by serum neutralization. Journal of Medical Virology 5:231–237
     [Google Scholar]
  4. Bridger J. C., Brown J. F. 1981; Development of immunity to porcine rotavirus in piglets protected from disease by bovine colostrum. Infection and Immunity 31:906–910
     [Google Scholar]
  5. Bridger J. C., Woode G. N. 1975; Neonatal calf diarrhoea: identification of a reovirus-like (rotavirus) agent in faeces by immunofluorescence and immune electron microscopy. British Veterinary Journal 131:528–535
     [Google Scholar]
  6. Bridger J. C., Brown J. F., Arnold P. S., Pocock D. H., McCrae M. A. 1983; Antigenic variation in porcine and fjovine rotaviruses. Fourth International Symposium on Neonatal Diarrhoea p 48 Canada: VIDO;
     [Google Scholar]
  7. Dennis M. J., Davies D. C., Hoare M. N. 1976; A simplified apparatus for the microbiological isolation of calves. British Veterinary Journal 132:642–646
     [Google Scholar]
  8. Estes M. K., Graham D. Y. 1980; Identification of rotaviruses of different origins by the plaque-reduction test. American Journal of Veterinary Research 41:151–152
     [Google Scholar]
  9. Flewett T. H., Woode G. N. 1978; The rotaviruses. Archives of Virology 57:1–23
     [Google Scholar]
  10. Gaul S. K., Simpson T. F., Woode G. N., Fulton R. W. 1982; Antigenic relationships among some animal rotaviruses: virus neutralization in vitro and cross-protection in piglets. Journal of Clinical Microbiology 16:495–503
     [Google Scholar]
  11. Greenberg H. B., Kalica A. R., Wyatt R. G., Jones R. W., Kapikian A. Z., Chanock R. M. 1981; Rescue of non-cultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus. Proceedings of the National Academy of Sciences, U,. S,. A 78:420–424
     [Google Scholar]
  12. Hall G. A., Bridger J. C., Chandler R. L., Woode G. N. 1976; Gnotobiotic piglets experimentally infected with neonatal calf diarrhoea reovirus-like agent (rotavirus). Veterinary Pathology 13:197–210
     [Google Scholar]
  13. Hoare M. N., Davies D. C., Hoare M. J. 1976; The derivation of gnotobiotic calves by a hysterotomy and slaughter technique. British Veterinary Journal 132:369–373
     [Google Scholar]
  14. Hoshino Y., Wyatt R. G., Scott F. W., Appel M. J. 1982; Isolation and characterization of a canine rotavirus. Archives of Virology 72:113–125
     [Google Scholar]
  15. Hoshino Y., Wyatt R. G., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. 1983a; Isolation and characterization of an equine rotavirus. Journal of Clinical Microbiology 13:585–591
     [Google Scholar]
  16. Hoshino Y., Wyatt R. G., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. 1983b; Serological comparision of canine rotavirus with various simian and human rotaviruses by plaque reduction neutralization and hemagglutination inhibition test. Infection and Immunity 41:169–173
     [Google Scholar]
  17. Hoshino Y., Wyatt R. G., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. 1983c; Isolation, propagation and characterization of a second equine rotavirus serotype. Infection and Immunity 41:1031–1037
     [Google Scholar]
  18. Kapikian A. Z., Conant R. M., Hamparian V. V., Chanock R. M., Chapple P. J., Dick E. C., Fenters J. D., Gwaltney J. M. Jr, Hamre D., Holper J. C., Jordan W. S. Jr, Lennette E. H., Melnick J. L., Mogabgab W. J., Mufson M. A., Phillips C. A., Schieble J. H., Tyrrell D. A. J. 1967; Rhinoviruses: a numbering system. Nature, London 213:761–763
     [Google Scholar]
  19. Kapikian A. Z., Cline W. L., Greenberg H. B., Wyatt R. G., Kalica A. R., Banks C. E., James H. D. Jr, Flores J., Chanock R. M. 1981; Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens. Infection and Immunity 33:415–425
     [Google Scholar]
  20. McNulty M. S. 1978; Rotaviruses. Journal of General Virology 40:1–18
     [Google Scholar]
  21. McNulty M. S., Allan G. M., McFerran J. B. 1976; Isolation of a cytopathic calf rotavirus. Research in Veterinary Science 21:114–115
     [Google Scholar]
  22. McNulty M. S., Allan G. M., Todd D., McFerran J. B., McKillop E. R., Collins D. S., McCracken R. M. 1980; Isolation of rotaviruses from turkeys and chickens: demonstration of distinct serotypes and RNA electropherotypes. Avian Pathology 9:363–375
     [Google Scholar]
  23. Matsuno S., Fiasegawa A., Kalica A. R., Kono R. 1980; Isolation of a recombinant between simian and bovine rotaviruses. Journal of General Virology 48:253–256
     [Google Scholar]
  24. Murakami Y., Nishioka N., Hashiguchi Y., Kuniyasu C. 1983; Serotypes of bovine rotaviruses distinguished by serum neutralization. Infection and Immunity 40:851–855
     [Google Scholar]
  25. Pedley S., Bridger J. C., Brown J. F., McCrae M. A. 1983; Molecular characterization of rotaviruses with distinct group antigens. Journal of General Virology 64:2093–2101
     [Google Scholar]
  26. Sato K., Inaba Y., Miura Y., Tokuhisa S., Matumoto M. 1982; Antigenic relationships between rotaviruses from different species as studied by neutralization and immunofluorescence. Archives of Virology 73:45–50
     [Google Scholar]
  27. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring A. J. 1977; Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Archives of Virology 53:287–294
     [Google Scholar]
  28. Thouless M. E., Bryden A. S., Flewett T. H. 1978; Serotypes of human rotaviruses. Lancet 1:39
     [Google Scholar]
  29. Tzipori S. R., Makin T. J., Smith M. L. 1980; The clinical response of gnotobiotic calves, pigs and lambs to inoculation with human, calf, pig and foal rotavirus isolates. Australian Journal of Experimental Biology and Medical Sciences 58:309–318
     [Google Scholar]
  30. Urasawa S., Urasawa T., Taniguchi K. 1982; Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains. Infection and Immunity 38:781–784
     [Google Scholar]
  31. Woode G. N., Bridger J. C., Hall G., Dennis M. J. 1974; The isolation of a reovirus-like agent associated with diarrhoea in colostrum-deprived calves in Great Britain. Research in Veterinary Science 16:102–105
     [Google Scholar]
  32. Woode G. N., Bridger J. C., Hall G. A., Jones J. M., Jackson G. 1976; The isolation of reovirus-like agents (rotaviruses) from acute gastroenteritis of piglets. Journal of Medical Microbiology 9:203–209
     [Google Scholar]
  33. Woode G. N., Kelso N. E., Simpson T. F., Gaul S. K., Evans L. E., Babiuk L. 1983; Antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves. Journal of Clinical Microbiology 18:358–364
     [Google Scholar]
  34. Wyatt R. G., Mebus C. A., Yolken R. H., Kalica A. R., James H. D., Kapikian A. Z., Chanock R. M. 1979; Rotaviral immunity in gnotobiotic calves: heterologous resistance to human virus induced by bovine virus. Science 203:548–550
     [Google Scholar]
  35. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. 1982; Definition of human rotavirus serotypes by plaque reduction assay. Infection and Immunity 37:110–115
     [Google Scholar]
  36. Wyatt R. G., James H. D., Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. 1983; Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. Journal of Clinical Microbiology 18:310–317
     [Google Scholar]
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Antigenic and Pathogenic Relationships of Three Bovine Rotaviruses and a Porcine Rotavirus
J. Gen. Virol. 65, 1151 (1984); https://doi.org/10.1099/0022-1317-65-7-1151
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/content/journal/jgv/10.1099/0022-1317-65-7-1151
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