1887

Abstract

SUMMARY

The polypeptides soluble in 0.25 -HCl were extracted from the nuclei of BHK cells infected with herpes simplex virus type 1 or type 2 and separated by SDS-PAGE. Seventeen polypeptides were detectable in each extract of which 10 type 1 and nine type 2 polypeptides were reproducibly effectively extracted. In cells infected with bovine mammillitis virus, pseudorabies virus or equine herpesvirus type 1, at least 12, 13 and eight polypeptides respectively were acid-soluble. In addition to histones, three other cellular polypeptides were present in sizeable quantities in the acid extracts and could obscure other acid-soluble viral polypeptides. Possible relationships between some polypeptides of the five herpesviruses are discussed.

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1985-10-01
2024-04-16
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References

  1. Bayliss G. J., Marsden H. S., Hay J. 1975; Herpes simplex virus proteins: DNA-binding proteins in infected cells and in the virus structure. Virology 68:124–134
    [Google Scholar]
  2. Bookout J. B., Levy C. C. 1980; Comparative examination of the polypeptides of herpes simplex virus: types 1 and 2. Virology 101:198–216
    [Google Scholar]
  3. Castrucci G., Pedini B., Cilli V., Arancia G. 1972; Characterization of a viral agent resembling bovine herpes mammillitis virus. Veterinary Record 90:325–335
    [Google Scholar]
  4. Chantler J. K., Stevely W. S. 1973; Virus-induced proteins in pseudorabies-infected cells. Journal of Virology II:815–822
    [Google Scholar]
  5. Fenwick M. L., Walker M. J., Petkevich J. M. 1978; On the association of virus proteins with the nuclei of cells infected with herpes simplex virus. Journal of General Virology 39:519–529
    [Google Scholar]
  6. Freeman M. J., Powell K. L. 1982; DNA-binding properties of a herpes simplex virus immediate early protein. Journal of Virology 44:1084–1087
    [Google Scholar]
  7. Halliburton I. W. 1972; Biochemical comparisons of type 1 and type 2 herpes simplex viruses. In Oncogenesis and Herpesviruses 2 pp 432–438 Edited by M P., Biggs G, de Thé & Payne L. N. Lyon: IARC Scientific Publications;
    [Google Scholar]
  8. Halliburton I. W., Timbury M. C. 1973; Characterization of temperature-sensitive mutants of herpes simplex virus type 2. Growth and DNA synthesis. Virology 54:60–68
    [Google Scholar]
  9. Hay R. T., Hay J. 1980; Properties of herpesvirus-induced ‘immediate early’ polypeptides. Virology 104:230–234
    [Google Scholar]
  10. Honess R. W., Roizman B. 1973; Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell. Journal of Virology 12:1347–1365
    [Google Scholar]
  11. Killington R. A., Yeo J., Honess R. W., Watson D. H., Duncan B. E., Halliburton I. W., Mumford J. 1977; Comparative analyses of the proteins and antigens of five herpesviruses. Journal of General Virology 37:297–310
    [Google Scholar]
  12. Knipe D. M., Quinlan M. P., Spang A. E. 1982; Characterization of two conformational forms of the major DNA-binding protein encoded by herpes simplex virus 1. Journal of Virology 44:736–741
    [Google Scholar]
  13. La thangue N. B., Chan W. L. 1984; The characterization and purification of DNA binding proteins present within herpes simplex virus infected cells using monoclonal antibodies. Archives of Virology 79:13–33
    [Google Scholar]
  14. Littler E., Yeo J., Killington R. A., Purifoy D. J. M., Powell K. L. 1981; Antigenic and structural conservation of herpesvirus DNA-binding proteins. Journal of General Virology 56:409–419
    [Google Scholar]
  15. Marsden H. S., Crombie I. K., Subak-sharpe J. H. 1976; Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild-type and sixteen temperature-sensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  16. Mayr A., Bohm H. O., Brill J., Woyciechowska S. 1965; Charakterisierung eines Stutenabortvirus aus Polen und Vergleich mit bekannten Rhinopneumonitisvirus Stammen des Pferdes. Archiv fur die gesamte Virusforschung 17:216–230
    [Google Scholar]
  17. Powell K. L., Courtney R. J. 1975; Polypeptides synthesized in herpes simplex virus type 2 infected HEp-2 cells. Virology 66:217–228
    [Google Scholar]
  18. Powell K. L., Purifoy D. J. M. 1976; DNA-binding proteins of cells infected by herpes simplex virus type 1 and type 2. Intervirology 7:225–239
    [Google Scholar]
  19. Powell K. L., Littler E., Purifoy D. J. M. 1981; Non-structural proteins of herpes simplex virus II. The major virus-specific DNA binding protein. Journal of Virology 39:894–902
    [Google Scholar]
  20. Purifoy D. J. M., Powell K. L. 1976; DNA-binding proteins induced by herpes simplex virus type 2 in HEp-2 cells. Journal of Virology 19:717–731
    [Google Scholar]
  21. Stevens J. G., Kado-boll G. J., Haven C. B. 1969; Changes in nuclear basic proteins during pseudorabies virus infection. Journal of Virology 3:490–497
    [Google Scholar]
  22. Tyler S. J., Hay J., Stevely W. S. 1974; Nuclear protein synthesis in hamster kidney cells: the effect of pseudorabies virus infection. Archiv fur die gesamte Virusforschung 44:84–91
    [Google Scholar]
  23. Wilcox K. W., Kohn A., Sklyanskaya E., Roizman B. 1980; Herpes simplex virus phosphoproteins. I. Phosphate cycles on and off some virus polypeptides and can alter their affinity for DNA. Journal of Virology 33:167–182
    [Google Scholar]
  24. Yeo J., Killington R. A., Watson D. H., Powell K. L. 1981; Studies on cross-reactive antigens in the herpesviruses. Virology 108:256–266
    [Google Scholar]
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