1887

Abstract

SUMMARY

Bacteriophage c6A is a lytic phage that infects strains of . Infection of C6 under standard conditions yielded 124 ± 8 p.f.u. per infected cell after a latent period of 25 min at 30 °C. The virion of c6A was shown to contain at least 12 polypeptides and a 21.9 kilobase double-stranded, linear DNA genome with complementary 5′-protruding single-stranded termini. The (G + C) content of this DNA was estimated to be 36.7%. A restriction map was constructed which indicates that a number of restriction endonucleases did not digest the DNA and that others cleaved with a much lower frequency than expected.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-12-2737
1985-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/12/JV0660122737.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-12-2737&mimeType=html&fmt=ahah

References

  1. Boussemaer J. P., Schrauwen P. P., Sourrouille J. L., Guy P. 1980; Multiple modification/restriction systems in lactic streptococci and their significance in defining a phage-typing system. Journal of Dairy Research 47:401–409
    [Google Scholar]
  2. Heap H. A., Jarvis A. W. 1980; A comparison of prolate-and isometric-headed lactic streptococcal bacteriophages. New Zealand Journal of Dairy Science and Technology 15:75–81
    [Google Scholar]
  3. Jarvis A. W. 1984; Differentiation of lactic streptococcal phages into phage species by DNA–DNA homology. Applied and Environmental Microbiology 47:343–349
    [Google Scholar]
  4. Keogh B. P. 1973; Adsorption, latent period and burst size of phages of some strains of lactic streptococci. Journal of Dairy Research 40:303–309
    [Google Scholar]
  5. Keogh B. P., Shimmin P. D. 1974; Morphology of the bacteriophages of lactic streptococci. Applied Microbiology 27:411–415
    [Google Scholar]
  6. Kessler C., Neumaier P. S., Wolf W. 1985; Recognition sequences of restriction nucleases and methylases-a review. Gene 33:1–102
    [Google Scholar]
  7. Knittel M. D., Black C. H., Sandine W. E., Elliker P. R. 1965; A simple procedure for comparing compositional distribution of purified deoxyribonucleic acid samples. Bacteriological Proceedings abstract G9
    [Google Scholar]
  8. Kruger D. H., Bickle T. A. 1983; Bacteriophage survival: multiple mechanisms for avoiding the deoxyribonucleic acid restriction systems of their hosts. Microbiological Reviews 47:345–360
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Mandel M., Igambi L., Bergendahl J., Dodson M. L., Scheltgen E. 1970; Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. Journal of Bacteriology 101:333–338
    [Google Scholar]
  11. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Nicholls K. D., Holloway B. W. 1962; Latent periods and burst sizes of lactic streptococcal phages. Australian Journal of Dairy Technology 17:137–139
    [Google Scholar]
  13. Nyiendo J. A. 1975 Studies on host range, fine structure, and nucleic acids of lactic streptococcus bacteriophages Ph D. thesis Oregon State University;
    [Google Scholar]
  14. Nyiendo J., Seidler R. J., Sandine W. E., Elliker P. R. 1974; Preparation and storage of high-titer lactic streptococcus bacteriophages. Applied Microbiology 27:72–77
    [Google Scholar]
  15. Weber K., Pringle J. R., Osborn M. 1972; Measurement of molecular weights by electrophoresis on SDS–acrylamide gel. Methods in Enzymology 26:3–27
    [Google Scholar]
  16. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
    [Google Scholar]
  17. Yehle C. O. 1978; Genome-linked protein associated with the 5′ termini of bacteriophage 029 DNA. Journal of Virology 27:776–783
    [Google Scholar]
  18. Yoshikawa H., Ito J. 1981; Terminal proteins and short inverted repeats of the small Bacillus bacteriophage genomes. Proceedings of the National Academy of Sciences, U.S.A 78:2596–2600
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-12-2737
Loading
/content/journal/jgv/10.1099/0022-1317-66-12-2737
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error