1887

Abstract

SUMMARY

The mechanism of resistance of murine macrophages (M) to infection by herpes simplex virus type 1 (HSV-1) was examined. Infection of bone marrow-derived M (BMDM) and resident peritoneal M (Res-M) was compared with infection of permissive Vero cells. In contrast to HSV-1 infection in Vero cells, no infectious virus was produced from either M cell type. However, marked cytopathic effect (c.p.e.) was evident in BMDM at 48 h post-infection, while there was no c.p.e. at any time post-infection in the Res-M. Cloned RI subgenomic fragments representing the entire HSV-1 genome were used as probes in DNA:DNA hybridization experiments to determine the viral genome content in the infected cell types. In Res-M, HSV-1 DNA was present at early times post-infection but declined rapidly. In BMDM, the virus genome was always detected and increased with time after infection. The results suggest that Res-M restrict HSV-1 production at a point prior to viral DNA synthesis, whereas the block in HSV production in BMDM occurs at a later stage in the viral replicative cycle.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-5-1123
1985-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/5/JV0660051123.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-5-1123&mimeType=html&fmt=ahah

References

  1. Alwine J. C., Kemp D. J., Parker B. A., Reiser J., Renart J., Strak G. R., Wahl G. M. 1980; Detection of specific RNAs or specific fragments of DNA by fractionization in gels and transfer to diazobenzyloxymethyl paper. Methods in Enzymology 68:220–242
    [Google Scholar]
  2. Dempsey W. 1984 Federation Proceedings 43:1434
    [Google Scholar]
  3. Dutko F., Oldstone M. B. A. 1981; Cytomegalovirus causes a latent infection in undifferentiated cells and is activated by induction of cell differentiation. Journal of Experimental Medicine 159:1636–1651
    [Google Scholar]
  4. Goldin A. L., Sandri-Goldin R. M., Levine M., Glorioso J. C. 1981; Cloning of herpes simplex virus type I sequences representing the whole genome. Journal of Virology 38:50–58
    [Google Scholar]
  5. Gonczol E., Andrews P. W., Plotkin P. A. 1984; Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224:159–161
    [Google Scholar]
  6. Leary J. J., Brigati B. J., Ward D. C. 1983; Rapid and sensitive colorimetric method for visualizing biotin-labelled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: bio-blots. Proceedings of the National Academy of Sciences, U. S. A 80:4045–4049
    [Google Scholar]
  7. Linnavuori K., Hovi T. 1983; Restricted replication of herpes simplex virus in human monocyte cultures: role of interferon. Virology 130:1–9
    [Google Scholar]
  8. Mogensen S. C. 1979; Role of macrophages in natural resistance to virus infections. Microbiological Reviews 43:1–26
    [Google Scholar]
  9. Morahan P. S. 1981; Macrophage nomenclature and tables of characteristics. Journal of the Reticuloendothelial Society 27:223–245
    [Google Scholar]
  10. Morahan P. S. 1984; Interactions of herpes viruses with mononuclear phagocytes. In Immunobiology of Herpes Simplex Virus Injection pp 71–89 Edited by Rouse B. T., Lopez C. Boca Raton: CRC Press;
    [Google Scholar]
  11. Morafian P. S., Breinig M. C., McGeorge M. B. 1977; Immune responses to vaginal or systemic infection of Balb/c mice with herpes simplex virus type 2. Journal of Immunology 119:2030–2036
    [Google Scholar]
  12. Morahan P. S., Edelson P. J., Gass K. 1980; Changes in macrophage ectoenzymes associate with anti-tumor activity. Journal of Immunology 125:1312–1317
    [Google Scholar]
  13. Morahan P. S., Connor I., Leary K. 1985; Virus and the versatile macrophage. British Medical Bulletin41 (in press)
    [Google Scholar]
  14. Peden K., Mounts P., Hayward G. S. 1982; Homology between mammalian cell DNA sequences and human herpes virus genomes detected by a hybridization procedure with high complexity probe. Cell 31:71–80
    [Google Scholar]
  15. Puga A., Cantin E. M., Notkins A. L. 1982; Homology between murine and human cellular DNA sequences and terminal repetition of S component of herpes simplex virus type 1 genome. Cell 31:81–87
    [Google Scholar]
  16. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  17. Sandri-Goldin R., Levine M., Glorioso J. C. 1981; Method for induction of mutations in physically defined regions of the herpes simplex virus genome. Journal of Virology 38:41–49
    [Google Scholar]
  18. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  19. Stevens J. G., Cook M. L. 1971; Restriction of herpes simplex virus by macrophages. An analysis of the cell-virus interaction. Journal of Experimental Medicine 133:19–38
    [Google Scholar]
  20. Stewart C. C. 1981; Murine mononuclear phagocytes from bone marrow. In Methods for Studying Mononuclear Phagocytes pp 5–20 Edited by Adams D. O., Koren H. S., Edelson P. J. New York: Academic Press;
    [Google Scholar]
  21. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U. S. A 77:5201–5205
    [Google Scholar]
  22. Wahl G. M., Stern M., Stark G. R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proceedings of the National Academy of Sciences, U S,. A 76:3683–3687
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-5-1123
Loading
/content/journal/jgv/10.1099/0022-1317-66-5-1123
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error