1887

Abstract

Summary

The phenomenon known as antibody-dependent enhancement (ADE) has been demonstrated but its significance in viral pathogenesis is uncertain even though it has been associated with dengue shock syndrome. Here we report for the first time the enhancement of virus virulence in mice using monoclonal antibodies (MAbs) prepared against yellow fever (YF) viruses. Our results show that the average survival time of mice was reduced by up to 33% (i.e. 6.7 to 4.5 days) and that ADE is both antibody dose-dependent and antibody- and virus strain-specific. A total of 12 YF viruses and 11 MAbs were examined and of these only three YF viruses (FNV, Asibi and B11) could be enhanced by only two MAbs (427 and 126). A particular combination of virus and antibody is required for ADE to take place.

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/content/journal/jgv/10.1099/0022-1317-67-11-2539
1986-11-01
2024-04-24
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References

  1. Brandt W. E., McCown J. M., Gentry M. K., Russell P. K. 1982; Infection enhancement of dengue type 2 virus in the U-937 human monocyte cell line by antibodies to flavivirus cross-reactive determinants. Infection and Immunity 36:1036–1041
    [Google Scholar]
  2. Buckley A., Gould E. A. 1985; Neutralization of yellow fever virus studied using monoclonal and polyclonal antibodies. Journal of General Virology 66:2523–2531
    [Google Scholar]
  3. Cammack N., Gould E. A. 1986a; Topographical analysis of epitope relationships on the envelope glycoprotein of yellow fever 17D vaccine and the wild type Asibi parent virus. Virology 150:333–341
    [Google Scholar]
  4. Cammack N., Gould E. A. 1986b; Antigenic analysis of yellow fever virus glycoproteins: use of monoclonal antibodies in enzyme-linked immunosorbent assays. Journal of Virological Methods 13:135–142
    [Google Scholar]
  5. Gould E. A., Buckley A., Cammack N., Barrett A. D. T., Clegg J. C. S., Ishak R., Varma M. G. R. 1985; Examination of the immunological relationships between flaviviruses using yellow fever virus monoclonal antibodies. Journal of General Virology 66:1369–1382
    [Google Scholar]
  6. Gould E. A., Buckley A., Barrett A. D. T., Cammack N. 1986; Neutralizing (54K) and non-neutralizing (54K. and 48K) monoclonal antibodies against structural and non-structural yellow fever virus proteins confer immunity in mice. Journal of General Virology 67:591–595
    [Google Scholar]
  7. Halstead S. B. 1979; In vivo enhancement of dengue virus infection in rhesus monkeys by passively transferred antibody. Journal of Infectious Diseases 140:527–533
    [Google Scholar]
  8. Halstead S. B. 1981; The pathogenesis of dengue: molecular epidemiology in infectious disease. American Journal of Epidemiology 114:632–648
    [Google Scholar]
  9. Halstead S. B., Porterfield J. S., O’Rourke E. J. 1980; Enhancement of dengue virus infection in monocytes by flavivirus antisera. American Journal of Tropical Medicine and Hygiene 29:638–642
    [Google Scholar]
  10. Halstead S. B., Larsen K., Kliks K., Peiris J. S. M., Cardosa J., Porterfield J. S. 1983; Comparison of P388D1 mouse macrophage cell line and human monocytes for assay of dengue-2 infection enhancing antibodies. American Journal of Tropical Medicine and Hygiene 32:157–163
    [Google Scholar]
  11. Halstead S. B., Venkateshan C. N., Gentry M. K., Larsen L. K. 1984; Heterogeneity of infection enhancement of dengue-2 strains by monoclonal antibodies. Journal of Immunology 132:1529–1532
    [Google Scholar]
  12. Peiris J. S. M., Porterfield J. S., Roehrig J. T. 1982; Monoclonal antibodies against the flavivirus West Nile. Journal of General Virology 58:283–289
    [Google Scholar]
  13. Porterfield J. S. 1982; Immunological enhancement and the pathogenesis of dengue haemorrhagic fever. Journal of Hygiene 89:355–364
    [Google Scholar]
  14. Porterfield J. S., Cardosa M. S. 1984; Host range and tissue tropisms: antibody-dependent mechanisms. In Concepts in Viral Pathogenesis pp 177–122 Edited by Notkins A. L., Oldstone M. B. A. New York: Springer-Verlag;
    [Google Scholar]
  15. Prabhakar B. S., Nathanson N. 1981; Acute rabies death mediated by antibody. Nature, London 290:590–591
    [Google Scholar]
  16. Schlesinger J. S., Brandriss M. W. 1983; 17D yellow fever virus infection of P388D1 cells mediated by monoclonal antibodies: properties of the macrophage Fc receptor. Journal of General Virology 64:1255–1262
    [Google Scholar]
  17. Weiss R. C., Scott F. W. 1981; Antibody mediated enhancement of disease in feline infectious peritonitis: comparisons with dengue haemorrhagic fever. Comparative Immunology, Microbiology and Infectious Disease 4:175–189
    [Google Scholar]
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