1887

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Volume 67, Issue 3

Molecular Cloning of Complementary DNA to Newcastle Disease Virus, and Nucleotide Sequence Analysis of the Junction between the Genes Encoding the Haemagglutinin-Neuraminidase and the Large Protein Free

Abstract

Summary

Complementary DNA clones to 90% of the Newcastle disease virus (NDV) genome have been produced and mapped. These clones cover the entire HN, F and M genes, most if not all of the L gene and parts of the NP and P genes. The map of overlapping clones gives the gene order 3′-NP-P-M-F-HN-L-5′ for NDV, identical to the gene order of Sendai virus, on the assumption that the NP gene of NDV is at the 3′ end of the genome as previously suggested by inactivation of NDV transcription by u.v. light. The nucleotide sequence of 453 bases covering the junction between the HN and L genes has been determined. There is nucleotide sequence homology to the consensus polyadenylation and mRNA start sites of Sendai virus and vesicular stomatitis virus. The deduced amino acid sequence of the C terminus of the HN protein of NDV shows homology to the C-terminal amino acid sequences of the HN proteins of simian virus 5 and Sendai virus. An explanation for the presence of HN, the precursor to HN in some strains of NDV, is suggested by the presence of a long non-coding region at the 3′ terminus of the mRNA encoding the HN protein of NDV that could, by mutation, allow synthesis of a larger polypeptide.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): molecular cloning , NDV , paramyxovirus and sequence determination
© The Journal of General Virology 1986
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1986-03-01
2024-04-20
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References

  1. Ball L. A., Collins P. L., Hightower L. E. 1978; Transcription, translation, and mapping of the genes of Newcastle disease virus. In Negative Strand Viruses and the Host Cell pp. 367–382 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
     [Google Scholar]
  2. Biggin M. D., Gibson T. J., Hong G. F. 1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences, U.S.A. 80:3963–3965
     [Google Scholar]
  3. Birnboim H. C., Doly J. 1981; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
     [Google Scholar]
  4. Blumberg B. M., Gilorgi C., Rose K., Kolakofsky D. 1985a; Sequence determination of the Sendai virus fusion protein gene. Journal of General Virology 66:317–331
     [Google Scholar]
  5. Blumberg B., Giorgi C., Roux L., Rajin R., Dowling P., Chollet A., Kolakofsky D. 1985b; Sequence determination of the Sendai virus HN gene and its comparison to the influenza virus glycoproteins. Cell 41:269–278
     [Google Scholar]
  6. Cann A. J., Stanway G., Hauptmann R., Minor P. D., Schild G. C., Clark D. L., Mountford R. C., Almond J. W. 1983; Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins. Nucleic Acids Research 11:1267–1281
     [Google Scholar]
  7. Chambers P., Samson A. C. R. 1980; A new structural protein for Newcastle disease virus. Journal of General Virology 50:155–166
     [Google Scholar]
  8. Chambers P., Samson A. C. R. 1982; Non–structural proteins in Newcastle disease virus-infected cells. Journal of General Virology 58:1–12
     [Google Scholar]
  9. Chirgwin J. M., Przybyla A. E., Macdonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
     [Google Scholar]
  10. Choppin P. W., Compans R. W. 1975; Reproduction of paramyxoviruses. In Comprehensive Virology vol 4 pp. 95–178 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
     [Google Scholar]
  11. Clark A. J., Clissold P. M., Bishop J. O. 1982; Variation between mouse major urinary protein genes isolated from a single inbred line. Gene 18:221–230
     [Google Scholar]
  12. Collins P. L., Hightower L. E., Ball L. A. 1978; Transcription and translation of Newcastle disease virus mRNAs in vitro. Journal of Virology 28:324–336
     [Google Scholar]
  13. Collins P. L., Hightower L. E., Ball L. A. 1980; Transcriptional map for Newcastle disease virus. Journal of Virology 35:682–693
     [Google Scholar]
  14. Collins P. L., Wertz G. W., Ball L. A., Hightower L. E. 1982; Coding assignments of the five smaller mRNAs of Newcastle disease virus. Journal of Virology 43:1024–1031
     [Google Scholar]
  15. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
     [Google Scholar]
  16. Dietzschold B., Wunner W. H., Wiktor T. J., Dwight Lopes A., Lafon M., Smith C. L., Koprowski H. 1983; Characterization of an antigenic determinant of the glycoprotein that correlates with pathogenicity of rabies virus. Proceedings of the National Academy of Sciences, U.S.A 80:70–74
     [Google Scholar]
  17. Dowling P. C., Giorgi C., Roux L., Dethlefsen L. A., Galanctowicz M. E., Blumberg B. M., Kolakofsky D. 1983; Molecular cloning of the 3′-proximal third of Sendai virus genome. Proceedings of the National Academy of Sciences, U.S.A 80:5213–5216
     [Google Scholar]
  18. Evans D. M. A., Dunn G., Minor P. D., Schild G. C., Cann A. J., Stanway G., Almond J. W., Currey K., Maizel J. V. JR 1985; Increased neurovirulence associated with a single nucleotide change in a noncoding region of the Sabin type 3 poliovaccine genome. Nature, London 314:548–550
     [Google Scholar]
  19. Fields S., Winter G., Brownlee G. G. 1981; Structure of the neuraminidase gene in human influenza virus A/PR/8/34. Nature, London 290:213–217
     [Google Scholar]
  20. Garten W., Kohama T., Klenk H.-D. 1980; Proteolytic activation of the haemagglutinin-neuraminidase of Newcastle disease virus involves loss of a glycopeptide. Journal of General Virology 51:207–211
     [Google Scholar]
  21. Giorgi C., Blumberg B. M., Kolakofsky D. 1983; Sendai virus contains overlapping genes expressed from a single mRNA. Cell 35:829–836
     [Google Scholar]
  22. Glazier K., Raghow R., Kingsbury D. W. 1977; Regulation of Sendai virus transcription: evidence for a single promoter in vivo. Journal of Virology 21:863–871
     [Google Scholar]
  23. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy oj Sciences, U.S.A. 72:3961–3965
     [Google Scholar]
  24. Gupta C., Kingsbury D. W. 1984; Complete sequences of the intergenic and mRNA start signals in the Sendai virus genome: homologies with the genome of vesicular stomatitis virus.. Nucleic Acids Research 12:38293841
     [Google Scholar]
  25. Hidaka Y., Kanda T., Iwasaki K., Nomoto A., Shioda T., Shibuta H. 1984; Nucleotide sequence of a Sendai virus genome region covering the entire M gene and the 3′ proximal 1013 nucleotides of the F gene. Nucleic Acids Research 12:7965–7973
     [Google Scholar]
  26. Hiebert S. W., Paterson R. G., Lamb R. A. 1985; Haemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor. Journal of Virology 54:1–6
     [Google Scholar]
  27. Hightower L. E., Bratt M. A. 1974; Protein synthesis in Newcastle disease virus infected chick embryo cells. Journal of Virology 13:788–800
     [Google Scholar]
  28. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural difference of Sendai viruses grown in eggs and tissue culture cells. Journal of Virology 12:1457–1465
     [Google Scholar]
  29. Klenk H. -D., Choppin P. W. 1969; Lipids of plasma membranes of monkey and hamster kidney cells and of parainfluenza virions grown in these cells. Virology 30:255–268
     [Google Scholar]
  30. Klenk H. -D., Choppin P. W. 1970; Glycosphingolipids of plasma membranes of cultured cells and an enveloped virus (SV5) grown in these cells. Proceedings of the National Academy of Sciences, U. S. A. 66:57–64
     [Google Scholar]
  31. Kolakofsky D., Bruschi A. 1975; Antigenomes in Sendai virions and Sendai virus-infected cells. Virology 66:185–191
     [Google Scholar]
  32. Kolakofsky D., Boy De La Tour E., Bruschi A. 1974a; Self-annealing of Sendai virus RNA. Journal of Virology 14:33–39
     [Google Scholar]
  33. Kolakofsky D., Boy De La Tour E., Delius H. 1974b; Molecular weight determination of Sendai and Newcastle disease virus RNA. Journal of Virology 13:261–268
     [Google Scholar]
  34. Kurilla M. G., Stone H. O., Keene J. D. 1985; RNA sequence and transcriptional properties of the 3′ end of the Newcastle disease virus genome. Virology 145:203–212
     [Google Scholar]
  35. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
     [Google Scholar]
  36. McDonnell M. W., Simon M. N., Studier F. W. 1977; Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology 110:119–146
     [Google Scholar]
  37. Mcgeoch D. J. 1979; Structure of the gene N: NS intercistronic junction in the genome of vesicular stomatitis virus. Cell 17:673–681
     [Google Scholar]
  38. Mackett M., Smith G. L., Moss B. 1985; The construction and characterization of vaccinia virus recombinants expressing foreign genes. In DNA Cloning: A Practical Approach pp. 191–211 Edited by Glover D. M. Oxford: IRL Press;
     [Google Scholar]
  39. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  40. Merz D. C., Scheid A., Choppin P. W. 1981; Immunological studies of the functions of paramyxovirus glycoproteins. Virology 109:94–105
     [Google Scholar]
  41. Messing J., Vieira J. 1982; A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269–276
     [Google Scholar]
  42. Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. 1970; Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus and Sendai virus. Journal of Virology 6:677–684
     [Google Scholar]
  43. Nagai Y., Klenk H.-D. 1977; Activation of precursors to both glycoproteins of NDV by proteolytic cleavage. Virology 77:125–134
     [Google Scholar]
  44. Nagai Y., Klenk H.-D., Rott R. 1976; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
     [Google Scholar]
  45. Paoletti E., Lipinskas B. R., Samsonoff C., Mercer S., Panicalli D. 1984; Construction of live vaccines using genetically engineered poxviruses: biological activity of vaccinia virus recombinants expressing the hepatitis B surface antigen and the herpes simplex virus glycoprotein D. Proceedings of the National Academy of Sciences, U.S.A. 81:193–197
     [Google Scholar]
  46. Paterson R. G., Harris T. J. R., Lamb R. A. 1984; Fusion protein of the paramyxovirus simian virus 5: nucleotide sequence of mRNA predicts a highly hydrophobic glycoprotein. Proceedings of the National Academy of Sciences, U.S.A. 81:6706–6710
     [Google Scholar]
  47. Porter A. G., Barber C., Carey N. H., Hallewell R. A., Threlfall G., Emtage J. S. 1979; Complete nucleotide sequence of an influenza virus haemagglutinin gene from cloned DNA. Nature, London 282:471–477
     [Google Scholar]
  48. Rave N., Crkvenjakov R., Boedtker H. 1979; Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Research 6:3559–3567
     [Google Scholar]
  49. Robinson W. S. 1970; Self-annealing of subgroup 2 myxovirus RNAs. Nature, London 225:944–945
     [Google Scholar]
  50. Rose J. K. 1980; Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus. Cell 19:415–421
     [Google Scholar]
  51. Rott R., Klenk H.-D. 1977; Structure and assembly of viral envelopes. Cell Surface Reviews 2:47–81
     [Google Scholar]
  52. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
     [Google Scholar]
  53. Schuy W., Garten W., Linder D., Klenk H.-D. 1984; The carboxyterminus of the haemagglutinin- neuraminidase of Newcastle disease virus is exposed at the surface of the viral envelope. Virus Research 1:415–426
     [Google Scholar]
  54. Shimizu K., Ishida N. 1975; The smallest protein of Sendai virus: its candidate function of binding nucleocapsid to envelope. Virology 67:427–437
     [Google Scholar]
  55. Shioda T., Hidaka Y., Kanda T., Shibuta H., Namota A., Kentaro I. 1983; Sequence of 3, 687 nucleotides from the 3′ end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins. Nucleic Acids Research 11:7317–7330
     [Google Scholar]
  56. Smith G. L., Mackett M., Moss B. 1983; Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen. Nature, London 302:490–495
     [Google Scholar]
  57. Smith G. W., Hightower L. E. 1981; Identification of the P protein and other disulphide-linked and phosphorylated proteins of Newcastle disease virus. Journal of Virology 37:256–267
     [Google Scholar]
  58. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
     [Google Scholar]
  59. Staden R. 1984; Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Research 12:521–538
     [Google Scholar]
  60. Thomas G. P., Barry R. D., Fellner P., Smith J. 1978; Newcastle disease virus messenger RNAs. In Negative Strand Viruses and the Host Cell pp. 381–394 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
     [Google Scholar]
  61. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A. 77:5201–5205
     [Google Scholar]
  62. Waterson A. P., Pennington T. H., Allan W. H. 1967; Virulence in Newcastle disease virus. A preliminary study. British Medical Bulletin 23:138–143
     [Google Scholar]
  63. Wilde A., Morrison T. 1984; Structural and functional characterization of Newcastle disease virus polycistronic RNA species. Journal of Virology 51:71–76
     [Google Scholar]
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Molecular Cloning of Complementary DNA to Newcastle Disease Virus, and Nucleotide Sequence Analysis of the Junction between the Genes Encoding the Haemagglutinin-Neuraminidase and the Large Protein
J. Gen. Virol. 67, 475 (1986); https://doi.org/10.1099/0022-1317-67-3-475
/content/journal/jgv/10.1099/0022-1317-67-3-475
/content/journal/jgv/10.1099/0022-1317-67-3-475
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