Recognition of Influenza A Virus Nucleoprotein by Human Cytotoxic T Lymphocytes
McMichael, Andrew J. and Michie, Colin A. and Gotch, Frances M. and Smith, Geoffrey L. and Moss, Bernard,, 67, 719-726 (1986),
doi = https://doi.org/10.1099/0022-1317-67-4-719,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Summary A recombinant vaccinia virus (NP-VAC) containing cDNA corresponding to segment 5, the nucleoprotein (NP) gene of influenza A/PR/8/34 virus was used to examine the specificity of human influenza virus immune cytotoxic T lymphocytes (CTL). Effector cell preparations from two donors recognized autologous lymphocytes that had been infected with NP-VAC. Lysis was specific because cells infected with vaccinia virus were not killed and recognition was HLA-restricted. In one donor, the influenza virus-specific CTL response changed with time so that his effector cells no longer recognized autologous lymphocytes infected with NP-VAC. However, a component that was NP-specific remained because these CTL lysed the more sensitive autologous B lymphoblastoid cells that had been infected with NP-VAC. In four other donors, no NP-specific CTL response could be detected using autologous lymphocyte targets. Thus NP, an internal virus protein, is one antigen that is recognized by human influenza A virus-specific CTL, but it is likely that other individual virus components contribute to the total CTL response.,
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