1887

Abstract

Summary

Monoclonal antibodies to the haemagglutinin (HA) of the avian H1 influenza virus A/duck/Alberta/35/76 were used to construct an operational antigenic map of the HA molecule and to study the interrelationships of H1 viruses from different hosts. Haemagglutination inhibition tests between the monoclonal antibodies and variants selected by them provided evidence of four antigenic regions which overlap to varying degrees. Avian H1 influenza viruses displayed a spectrum of reactivities to the monoclonal antibody panel. Representatives of the epidemic strains of human H1 influenza viruses and early swine influenza viruses showed little or no reactivity with the monoclonal antibodies but swine influenza-like viruses isolated from pigs and humans in the last decade reacted with 11 of 17 antibodies. The antigenic similarity of these viruses to many avian isolates suggests that there has been a transfer of HA genetic information between mammalian and avian H1 influenza viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-6-983
1986-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/6/JV0670060983.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-6-983&mimeType=html&fmt=ahah

References

  1. Aymard-Henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schild G. C., Webster R. G. 1973; Influenzavirus neuraminidase and neuraminidase-inhibition test procedures. Bulletin of the World Health Organization 48:199–202
    [Google Scholar]
  2. Caton A. J., Brownlee G. G., Yewdell J. W., Gerhard W. 1982; The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype). Cell 31:417–427
    [Google Scholar]
  3. Caton A. I., Raymond F. L., Brownlee G. G., Yewdell I. W., Gerhard W. 1983; Antigenic variation in influenza virus. Transactions. Biochemical Society 11:435–441
    [Google Scholar]
  4. Easterday B. C. 1975; Animal influenza. In The Influenza Viruses and Influenza pp 449–481 Edited by Kilbourne E. D. New York: Academic Press;
    [Google Scholar]
  5. Gerhard W., Webster R. G. 1978; Antigenic drift in influenza A viruses. I. Selection and characterization of antigenic variants of A/PR/8/34 (H0N1) influenza virus with monoclonal antibodies. Journal of Experimental Medicine 148:383–392
    [Google Scholar]
  6. Gerhard W., Yewdell J., Frankel M. E., Webster R. 1981; Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies. Nature, London 290:713–717
    [Google Scholar]
  7. Hinshaw V. S., Bean W. J., Webster R. G., Easterday B. C. 1978; The prevalence of influenza viruses in swine and the antigenic and genetic relatedness of influenza viruses from man and swine. Virology 84:51–62
    [Google Scholar]
  8. Hinshaw V. S., Webster R. G., Easterday B. C., Bean W. J. 1981; Replication of avian influenza A viruses in mammals. Infection and Immunity 34:354–361
    [Google Scholar]
  9. Hinshaw V. S., Webster R. G., Bean W. J., Downie J., Senne D. A. 1983; Swine influenza-like viruses in turkeys: potential source of virus for humans?. Science 220:206–208
    [Google Scholar]
  10. Hinshaw V. S., Alexander D. J., Aymard M., Bachmann P. A., Easterday B. C., Flannoun C., Kida H., Lipkind M., Mackenzie J. S., Nerome K., Schild G. C., Scholtissek C., Senne D. A., Shortridge K. F., Skehel J. J., Webster R. G. 1984; Antigenic comparisons of swine-influenza-like H1N1 isolates from pigs, birds, and humans: an international collaborative study. Bulletin of the World Health Organization 62:871–878
    [Google Scholar]
  11. Kendal A. P., Noble G. R., Skehel J. J., Dowdle W. R. 1978; Antigenic similarity of influenza A (H1N1) viruses from epidemics in 1977–1978 to “Scandinavian” strains isolated in epidemics of 1950–1951. Virology 89:632–636
    [Google Scholar]
  12. Kida H., Brown L. E., Webster R. G. 1982; Biological activity of monoclonal antibodies to operationally defined antigenic regions on the hemagglutinin molecule of A/Seal/Massachusetts/1/80 (H7N7) influenza virus. Virology 122:38–47
    [Google Scholar]
  13. Kilbourne E. D. 1978; Genetic dimorphism in influenza viruses: characterization of stably associated hemagglutinin mutants differing in antigenicity and biological properties. Proceedings of the National Academy of Sciences, U.S.A. 75:6258–6262
    [Google Scholar]
  14. Köhler G., Milstein C. 1976; Derivation of specific antibody-producing tissue culture and tumour lines by cell fusion. European Journal of Immunology 6:511–519
    [Google Scholar]
  15. Laver W. G. 1969; Purification of influenza virus. In Fundamental Techniques in Virology pp 82–86 Edited by Habel K., Salzman N. P. New York: Academic Press;
    [Google Scholar]
  16. Nakajima S., Nakajima K., Kendal A. P. 1983; Identification of the binding sites to monoclonal antibodies on A/USSR/90/77 (H1N1) hemagglutinin and their involvement in antigenic drift in H1N1 influenza viruses. Virology 131:116–127
    [Google Scholar]
  17. Neustadt R. E., Fineberg H. V. 1978 The Swine Flu Affair: Decision-Making on a Slippery Disease. Washington: U. S. Department of Health, Education and Welfare.;
    [Google Scholar]
  18. Palmer D. F., Coleman M. T., Dowdle W. R., Schild G. C. 1975 Advanced Laboratory Techniques for Influenza Diagnosis Immunology Series No 6 Washington: U.S. Department of Health, Education and Welfare;
    [Google Scholar]
  19. Raymond F. L., Caton A. J., Cox N. J., Kendal A. P., Brownlee G. G. 1983; Antigenicity and evolution amongst recent influenza viruses of H1N1 subtype. Nucleic Acids Research 11:7191–7203
    [Google Scholar]
  20. Schild G. C., Oxford J. S., De Jong J. C., Webster R. G. 1983; Evidence for host-cell selection of influenza virus antigenic variants. Nature, London 303:706–709
    [Google Scholar]
  21. Scholtissek C., Burger H., Bachmann P. A., Hannoun C. 1983; Genetic relatedness of hemagglutinins of the H1 subtype of influenza A viruses isolated from swine and birds. Virology 129:521–523
    [Google Scholar]
  22. Shulman M., Wilde C. D., Köhler G. 1978; A better cell line for making hybridomas secreting specific antibodies. Nature, London 276:269–270
    [Google Scholar]
  23. Tobita K., Sugiura A., Enomoto C., Furuyama M. 1975; Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Medical Microbiology and Immunology 162:9–14
    [Google Scholar]
  24. Webster R. G., Laver W. G. 1980; Determination of the number of nonoverlapping antigen areas on Hong Kong (H3N2) influenza virus hemagglutinin with monoclonal antibodies and the selection of variants with potential epidemiological significance. Virology 104:139–148
    [Google Scholar]
  25. Webster R. G., Hinshaw V. S., Neave C. W., Bean W. J. 1984; Pandemics and animal influenza viruses. In The Molecular Virology and Epidemiology of Influenza pp 39–59 Edited by Stuart-Harris C. H., Potter C. London: Academic Press;
    [Google Scholar]
  26. Wiley D. C., Wilson I. A., Skehel J. J. 1981; Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature, London 289:373–378
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-6-983
Loading
/content/journal/jgv/10.1099/0022-1317-67-6-983
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error