Immunoreactivity of a Synthetic Pentadecapeptide Corresponding to the N-Terminal Region of the Scrapie Prion Protein
Shinagawa, Morikazu and Munekata, Eisuke and Doi, Satoshi and Takahashi, Kaname and Goto, Hitoshi and Sato, Gihei,, 67, 1745-1750 (1986),
doi = https://doi.org/10.1099/0022-1317-67-8-1745,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Summary A pentadecapeptide with an amino acid sequence corresponding to the amino-terminal region of the scrapie prion protein was synthesized. Immunization of a rabbit with the peptide conjugated with ovalbumin induced specific antibodies. The antibodies reacted with all three of the major polypeptides in a proteinase K-treated fraction obtained from brains of mice infected with the Obihiro strain of scrapie agent. Some peptides in the proteinase-untreated fraction also shared antigenicity with the three major polypeptides. Specific polypeptides were also detected by the antiserum in a fraction prepared from spleens, but only two of the three major polypeptides were found and the amounts of the polypeptides were less than in brain.,
language=,
type=