RT Journal Article SR Electronic(1) A1 Sullivan, Veronica A1 Smith, Geoffrey L.YR 1987 T1 Expression and Characterization of Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein G (gG) by Recombinant Vaccinia Virus: Neutralization of HSV-1 Infectivity with Anti-gG Antibody JF Journal of General Virology, VO 68 IS 10 SP 2587 OP 2598 DO https://doi.org/10.1099/0022-1317-68-10-2587 PB Microbiology Society, SN 1465-2099, AB SUMMARY A recombinant vaccinia virus expressing herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) has been constructed. Cells infected with this recombinant virus (gG-VAC) synthesized glycosylated proteins of 48K, 57K and 61K mol. wt. that were recognized by anti-HSV-1 sera. Rabbits and mice vaccinated with the live recombinant virus produced antibodies that recognized 48K, 57K and 61K mol. wt. proteins in HSV-1-infected cells. The gG polypeptides were present on cytoplasmic and nuclear membranes during infection with both HSV-1 and recombinant vaccinia virus gG- VAC. The 57K and 61K mol. wt. gG polypeptides were present in purified HSV-1 virions and were targets for antibody-mediated complement-dependent virus neutralization., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-10-2587