1887

Abstract

SUMMARY

The host component of control of scrapie incubation period in the mouse is manifested largely through the action of the gene. Only one mouse strain (VM) has been found that is p7p7 (prolonged incubation for ME7 agent) and two other strains have been derived from VM. All other strains, designated s7s7, have a short incubation for ME7. In the present study, the I strain was shown to fulfil the criteria that are characteristic of mouse strains with the p7 allele of (i) a comparatively long incubation period for ME7 and a short incubation period for 22A, (ii) the incubation period for F, hybrid mice (s7s7 x p7p7) either fell between the incubation periods for the parental strains (with ME7) or were longer than either parent (with 139A and 22A), (iii) amyloid plaques occurred following injection of ME7 and 87V but not after 22A or 139A, (iv) lesion profiles for four scrapie strains were similar in I mice and p7p7 mouse strains, and (v) injection of 87V led to disease in less than 300 days. Finally, allelism tests using F, hybrid mice (I × a p7p7 mouse strain) and progeny of backcrosses between these F, mice and I mice failed to reveal the segregation of additional major genes affecting scrapie incubation period.

Keyword(s): I mice , incubation period and scrapie
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-2-401
1987-02-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/2/JV0680020401.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-2-401&mimeType=html&fmt=ahah

References

  1. Bolton D. C., Mckinley M. P., Prusiner S. B. 1982; Identification of a protein that purifies with the scrapie prion. Science 218:1309–1311
    [Google Scholar]
  2. Bruce M. E., Dickinson A. G. 1979; Biological stability of dilferent classes of scrapie agent. In Slow Transmissible Diseases of the Nervous System 2 pp. 71–86 Prusiner S. B., Hadlow W. J. Edited by New York: Academic Press;
    [Google Scholar]
  3. Bruce M. E., Dickinson A. G. 1985; Genetic control of amyloid plaque production and incubation period in scrapie-infected mice. Journal of Neuropathology and Experimental Neurology 44:285–294
    [Google Scholar]
  4. Carlson G. A., Goodman p., Kingsbury D. T., Prusiner S. B. 1986; Scrapie incubation time and prion protein genes are linked. Clinical Research 34, no:2–675A
    [Google Scholar]
  5. Carp R. I, Callhan S. M., Sersen E. A., Moretz R. C. 1984; Preclinical changes in weight of scrapie-infected mice as a function of scrapie agent-mouse strain combination. Intervirology 21:61–69
    [Google Scholar]
  6. Carp R. I., Merz P. A., Moretz R. C., Somerville R. A., Callahan S. M., Wisniewiskl H. M. 1985; Biological properties of scrapie: an unconventional slow virus. In Subviral Pathogens of Plants and Animals: Viroids and Prions pp. 425–464 Maramorosch K., Mckelvey J. J. Edited by New York: Academic Press;
    [Google Scholar]
  7. Chesebro B., Race R., Wehrly K., Nishio J., Bloom M., Lechner D., Bergstrom S., Robbins K., Mayer L., Keith j. M., Garon c., Haase A. 1985; Identification of scrapie prion protein-specific mRNA in scrapie- infected and uninfected brain. Nature; London: 315331–333
    [Google Scholar]
  8. Dickinson A. G. 1975; Host-pathogen interactions in scrapie. Genetics 79:387–395
    [Google Scholar]
  9. Dickinson A. G., Fraser H. 1977; Scrapie pathogenesis in inbred mice: an assessment of host control and response involving many strains of agent. In Slow Virus Infections of the Central Nervous System pp. 3–14 Ter Meulen V., Katz M. Edited by New York: Springer-Verlag;
    [Google Scholar]
  10. Dickinson A. G., Meikle V. M. H. 1969; A comparison of some biological characteristics of the mouse-passaged scrapie agents, 22A and ME7. Genetical Research 13:213–225
    [Google Scholar]
  11. Dickinson A. G., Meikle V. M. H. 1971; Host genotype and agent effects in scrapie incubation: change in allelic interaction with different strains of agent. Molecular and General Genetics 112:73–79
    [Google Scholar]
  12. Dickinson A. G., Outram G. W. 1979; The scrapie replication site hypothesis and its implications for pathogenesis. In Slow Transmissible Diseases of the Nervous System 2 pp. 13–32 Prusiner S. B., Hadlow W. J. Edited by New York: Academic Press;
    [Google Scholar]
  13. Dickinson A. G., Outram G. W. 1983; Operational limitations in the characterization of the infective units of scrapie. In Virus Non Conventionnels et Affections du Systeme Nerveux Central, pp. 3–16 Court L. A., Cathala F. Edited by Paris: Masson;
    [Google Scholar]
  14. Dickinson A. G., Bruce M. E., Fraser H., Kimberlin R. H. 1984; Scrapie strain differences: the implications of stability and mutation. In Proceedings of Workshop on Slow Transmissible Diseases pp. 105–118 Tokyo: Japanese Ministry of Health and Welfare;
    [Google Scholar]
  15. Festing M. F. W. 1979 Inbred Strains in Biomedical Research London: Macmillan;
    [Google Scholar]
  16. Fraser H. 1971 Scrapie: the experimental disease in inbred strains of mice Ph.D. thesis University of Edinburgh:
    [Google Scholar]
  17. Fraser H. 1976; The pathology of natural and experimental scrapie. In Slow Virus Diseases of Animals and Man pp. 267–305 Kimberlin R. H. Amsterdam; North-Holland:
    [Google Scholar]
  18. Fraser H., Dickinson A. G. 1973; Scrapie in mice: agent-strain differences in the distribution and intensity of grey matter vacuolation. Journal of Comparative Pathology 83:29–40
    [Google Scholar]
  19. Kingsbury D. T., Kasper K. C., Stites D. P., Watson J. D., Hogan R. N., Prusiner S. B. 1983; Genetic Control of scrapie and Creutzfeldt-Jakob disease in mice. Journal of Immunology 131:491–196
    [Google Scholar]
  20. Merz P. A., Somerville R. A., Wisniewski H. M., Iqbal K. 1981; Abnormal fibrils from scrapie-infected brain. Acta neuropathologica 54:63–74
    [Google Scholar]
  21. Oesch B., Westaway D., Walchli M., Mckinley M. P., Kent S.B.H., Aebersold R., Barry R. A., Tempst P., Teplow D. B., Hood L. E., Prusiner S. B., Weissmann C. 1985; A cellular gene encodes scrapie PrP 27–30 protein. Cell 40:735–746
    [Google Scholar]
  22. Outram G. W. 1976; The pathogenesis of scrapie in mice. In Slow Virus Diseases of Animals and Man pp. Kimberlin R. H. Edited by Amsterdam: North-Holland;
    [Google Scholar]
  23. Robakis N. K., Sawh P. R., Wolfe G. C., Rubenstein R., Carp R. I., Innis M. A. 1986; Isolation of a cDN A clone encoding the leader peptide of prion protein and expression of the homologous gene in various tissues. Proceedings of the National Academy of Sciences, U.S.A 83:6377–6381
    [Google Scholar]
  24. Scott J. S., Dickinson A. G. 1985; A view of dementia from the standpoint of unconventional infections. Gerontologia biomedica acta 1:127–150
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-2-401
Loading
/content/journal/jgv/10.1099/0022-1317-68-2-401
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error