1887

Abstract

Summary

Monoclonal antibodies were produced against two distinct Thai dengue-2 (DEN-2) virus strains isolated in 1980 from dengue haemorrhagic fever patients. Nine of 36 hybridomas produced monoclonal IgG antibodies which reacted in radioimmune precipitation assays with the NS1 non-structural protein (42000 mol. wt.) from DEN-2- infected C6/36 () cells. The virus specificity of NS 1 -reactive monoclonal antibodies was determined by indirect immunofluorescence assays using LLC-MK2 cells infected with either the Thai 1980 DEN-2 isolates, prototype DEN viruses (four serotypes), Japanese encephalitis (JE), Murray Valley encephalitis, West Nile, Wesselbron or Tembusu viruses. Eight of the monoclonal antibody preparations were DEN-2-serotype specific. One preparation defined a special serological relationship between DEN-2 and JE viruses. Four preparations had detectable complement fixation titres using Thai DEN-2 virus antigen. Six spatially unique epitopes were identified using competitive binding assays.

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1987-03-01
2024-03-29
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References

  1. Bonner W. M. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Brandt W. E., Buescher E. L., Hetrick F. M. 1967; Production and characterization of arbovirus antibody in mouse ascitic fluid. American Journal of Tropical Medicine and Hygiene 16:339–347
    [Google Scholar]
  3. CENTERS FOR DISEASE CONTROL 1981 A Guide to the Performance of the Standardized Diagnostic Complement Fixation Method and Adaptation to Micro Test Revised February 1981 Atlanta: Centers for Disease Control;
    [Google Scholar]
  4. Clarke D. H., Casals J. 1958; Techniques for hemagglutination and hemagglutination inhibition with arthopod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  5. Falker W. A., Diwan A. R., Halstead S. B. 1973; Human antibody to dengue soluble complement-fixing (SCF) antigens. Journal of Immunology 111:1804–1809
    [Google Scholar]
  6. Gentry M. K., Henchal E. A., Mccown J. M., Brandt W. E., Dalrymple J. M. 1982; Identification of distinct antigenic determinants on dengue-2 virus by using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 31:548–555
    [Google Scholar]
  7. Gould E. A., Buckley A., Barrett A. D. T., Cammack N. 1986; Neutralizing (54K) and non-neutralizing (54K and 48K) monoclonal antibodies against structural and non-structural yellow fever virus proteins confer immunity in mice. Journal of General Virology 67:591–595
    [Google Scholar]
  8. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of125I-labelled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  9. Heinz F. X. 1986; Epitope mapping of flavivirus glycoproteins. Advances in Virus Research 31:103–168
    [Google Scholar]
  10. Heinz F. X., Mandl C., Berger R., Tuma W., Kunz C. 1984; Antibody-induced conformational changes result in enhanced avidity of antibodies to different antigenic sites on the tick-borne encephalitis virus glycoprotein. Virology 133:25–34
    [Google Scholar]
  11. Henchal E. A., Gentry M. K., Mccown J. M., Brandt W. E. 1982; Dengue virus-specific and flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. American Journal of Tropical Medicine and Hygiene 31:830–836
    [Google Scholar]
  12. Henchal E. A., Mccown I. M., Burke D. S., Seguin M. C., Brandt W. E. 1985; Epitopic analysis of antigenic determinants on the surface of dengue-2 virions using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 34:162–169
    [Google Scholar]
  13. Hengartner H., Luzzati A. L., Schreier M. 1978; Fusion of in vitro immunized lymphoid cells with X63Ag8. Current Topics in Microbiology and Immunology 81:92–99
    [Google Scholar]
  14. Kennett R. H., Denis K. A., Tung A. S., Klinman N. R. 1978; Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells. Current Topics in Microbiology and Immunology 81:77–91
    [Google Scholar]
  15. Kimura-Kuroda J., Yasui K. 1983; Topographical analysis of antigenic determinants on envelope glycoprotein V3 (E) of Japanese encephalitis virus using monoclonal antibodies. Journal of Virology 45:124–132
    [Google Scholar]
  16. Köhler G., Milstein C. 1976; Derivation of specific antibody-producing tissue culture and tumour cell lines by cell fusion. European Journal of Immunology 6:511–519
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  18. Qureshi A. A., Trent D. W. 1973; Group B arbovirus structural and nonstructural antigens. III. Serological specificity of solubilized intracellular viral proteins. Infection and Immunity 8:993–999
    [Google Scholar]
  19. Rice C. M., Lenches E. M., Eddy S. R., Shin S. J., Sheets R. L., Strauss J. H. 1985; Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229:726–733
    [Google Scholar]
  20. Roehrig J. T., Mathews J. H., Trent D. W. 1983; Identification of epitopes on the E glycoprotein of St. Louis encephalitis virus using monoclonal antibodies. Virology 128:118–126
    [Google Scholar]
  21. Russell P. K., Nisalak A., Sukhavachana P., Vivona S. 1967; A plaque reduction test for dengue virus neutralizing antibodies. Journal of Immunology 99:285–290
    [Google Scholar]
  22. Russell P. K., Chiewsilp D., Brandt W. E. 1970; Immunoprecipitation analysis of soluble complement-fixing antigens of dengue virus. Journal oflmmunology 105:838–845
    [Google Scholar]
  23. Schlesinger J. J., Walsh E. E., Brandriss M. W. 1984; Analysis of 17D yellow fever virus envelope protein epitodes using monoclonal antibodies. Journal of General Virology 65:1637–1644
    [Google Scholar]
  24. Schlesinger J. J., Brandriss M. W., Walsh E. E. 1985; Protection against 17D yellow fever encephalitis in mice by passive transfer of monoclonal antibodies to the nonstructural glycoprotein gp48 and by active immunization with gp48. Journal of Immunology 135:2805–2809
    [Google Scholar]
  25. Smith G. W., Wright P. I. 1985; Synthesis of proteins and glycoproteins in dengue type 2 virus-infected Vero and Aedes albopictus cells. Journal of General Virology 66:559–571
    [Google Scholar]
  26. Wechsler S. L., Rustigian R., Stallcup K. C., Byers K. B., Winston S. H., Fields B. N. 1979; Measles virus- specified polypeptide synthesis in two persistently infected HeLa cell lines. Journal of Virology 31:677–684
    [Google Scholar]
  27. Wright P. I., Warr H. M., Westaway E. G. 1981; Synthesis of glycoproteins in cells infected by the flavivirus Kunjin. Virology 109:418–427
    [Google Scholar]
  28. Yewdell J. W., Gerhard W. 1981; Antigenic characterization of viruses by monoclonal antibodies. Annual Review of Microbiology 35:185–206
    [Google Scholar]
  29. Zollinger W. D., Dalrymple J. M., Artenstein M. S. 1976; Analysis of parameters affecting the solid phase radioimmune assay quantitation of antibody to meningococcal antigens. Journal of Immunology 117:1788–1798
    [Google Scholar]
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