1887

Abstract

Summary

A recombinant interferon (IFN) hybrid has been found to have a broad host-range of activity in an antiviral assay (plaque reduction of vesicular stomatitis virus) and also high efficacy as an antiviral agent in at least 12 different animal cell species. The IFN hybrid consists of amino acids 1 to 60 from HuIFN-B and amino acids 61 to 166 from HuIFN-D. The profile of cross-species activity of the IFN-B/D hybrid has been compared with that of HuIFN-F, and of the parents HuIFN-B and -D. When both IFN-B and -D were active in a cell species, the hybrid IFN had comparable or better activity than the more active parental IFN. The hybrid shared a broad cross-species activity with IFN-D. However, the IFN-B/D hybrid was 10-fold more active on human cells, 30-fold more active on rabbit cells, and 50-fold more active on mouse cells than IFN-D.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-3-945
1987-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/3/JV0680030945.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-3-945&mimeType=html&fmt=ahah

References

  1. Aguet M., Groebke M., Dreiding P. 1984; Various human interferon a subclasses cross-react with common receptors: their binding affinities correlate with their specific biological activities. Virology 132:211–216
    [Google Scholar]
  2. Horisberger M. A., De Staritzky K. 1985; Sensitivity of influenza A viruses to human interferons in human diploid cells. FEMS Microbiology Letters 29:207–210
    [Google Scholar]
  3. Meister A., Uze G., Mogensen K. E., Gresser I., Tovey M. G., Grütter M., Meyer F. 1986; Biological activities and receptor binding of two human recombinant interferons and their hybrids. Journal of General Virology 67:1633–1643
    [Google Scholar]
  4. Rehberg E., Kelder B., Hoal E. G., Pestka S. 1982; Specific molecular activities of recombinant and hybrid leukocyte interferons. Journal of Biological Chemistry 257:11497–11502
    [Google Scholar]
  5. Sen G. C. 1984; Biochemical pathways in interferon-action. Pharmacology and Therapeutics 24:235–257
    [Google Scholar]
  6. Stewart W. E.II 1981 In The InterJeron System pp 135–145 New York: Springer-Verlag;
    [Google Scholar]
  7. Stitz L., Schellekens H. 1980; Influence of input multiplicity of infection on the antiviral activity of interferon. Journal of General Virology 46:205–210
    [Google Scholar]
  8. Streuli M., Hall A., Boll W., Stewart W. E.II Nagata S., Wessman C. 1981; Target Cell Specificity of tWO species of human interferons-a produced in Escherichia coli and of hybrid molecules derived from them. Proceedings of the National Academy of Sciences U.S.A.: 782848–2852
    [Google Scholar]
  9. Taguchi F., Siddell S. G. 1985; Difference in sensitivity to interferon among mouse hepatitis viruses with high and low virulence for mice. Virology 147:41–48
    [Google Scholar]
  10. Torchio C., Trowbridge R. S. 1977; Ovine cells: their long term cultivation and susceptibility of visna virus. In Vitro 13:252–259
    [Google Scholar]
  11. Weck P. K., Apperson S., May L., Stebbing N. 1981a; Comparison of the antiviral activities of various cloned human interferon-a subtypes in mammalian cell cultures. Journal of General Virology 57:233–237
    [Google Scholar]
  12. Weck P. K., Apperson S., May L., Stebbing N., Gray P. W., Leung D., Shepard H. M., Goeddel D. V. 1981b; Antiviral activities of hybrids of two major human leukocyte interferons. Nucleic Acids Research 9:6153–6166
    [Google Scholar]
  13. Yelverton E., Leung D., Weck P., Gray P. W., Goeddel D. V. 1981; Bacterial synthesis of a novel human leukocyte interferon. Nucleic Acids Research 9:731–741
    [Google Scholar]
  14. Yilma T. 1983; Sensitivity of ovine choroid plexus cells to human and other animal interferons. Journal of General Virology 64:2013–2016
    [Google Scholar]
  15. Zoon K., Zurnedden D., Arnheiter H. 1982; Specific binding of human a interferon to a high affinity cell surface binding site on bovine kidney cells. Journal of Biological Chemistry 257:4695–4697
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-3-945
Loading
/content/journal/jgv/10.1099/0022-1317-68-3-945
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error