%0 Journal Article %A Nair, C. N. %T Dependence of Guanidine Sensitivity of Poliovirus Replication on the Concentration of Monovalent Cations in the Culture Medium %D 1987 %J Journal of General Virology, %V 68 %N 4 %P 1195-1198 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-68-4-1195 %K [K+]effect %K poliovirus replication %K guanidine inhibition %K [Na+] %I Microbiology Society, %X SUMMARY An earlier suggestion that guanidine may inhibit picornavirus replication by interfering with a monovalent cation-mediated event was tested by determining the effect of varying monovalent cation concentration in isotonic medium on the sensitivity of poliovirus replication in HeLa cells to 0·2 mm-guanidine. Lowering [Na+] in the medium to 50 mm had no effect on virus replication. It was found that the degree of inhibition of virus replication by 0·2 mm-guanidine was inversely related to [Na+] in the medium: 99·8 %, 99·1 %, 38% and 0% inhibition in the presence of 50, 75, 100 and 145 mm-Na+ respectively. Likewise, guanidine uptake by HeLa cells was also inversely related to [Na+] in the medium. On the other hand, lowering medium [Na+] to 50 or 75 mm resulted in reduced intracellular [Na+] and [K+]. The increased sensitivity of virus replication to guanidine in the presence of low Na+ medium could be abolished with excess K+ in such medium. Excess K+ in low Na+ medium restored intracellular [Na+] and reduced guanidine uptake. Thus, the increased sensitivity of poliovirus replication to guanidine in the presence of low Na+ medium correlated with reduced intracellular [Na+] and [K+] and elevated guanidine uptake. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-4-1195