Dependence of Guanidine Sensitivity of Poliovirus Replication on the Concentration of Monovalent Cations in the Culture Medium
Nair, C. N.,, 68, 1195-1198 (1987),
doi = https://doi.org/10.1099/0022-1317-68-4-1195,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= SUMMARY An earlier suggestion that guanidine may inhibit picornavirus replication by interfering with a monovalent cation-mediated event was tested by determining the effect of varying monovalent cation concentration in isotonic medium on the sensitivity of poliovirus replication in HeLa cells to 0·2 mm-guanidine. Lowering [Na+] in the medium to 50 mm had no effect on virus replication. It was found that the degree of inhibition of virus replication by 0·2 mm-guanidine was inversely related to [Na+] in the medium: 99·8 %, 99·1 %, 38% and 0% inhibition in the presence of 50, 75, 100 and 145 mm-Na+ respectively. Likewise, guanidine uptake by HeLa cells was also inversely related to [Na+] in the medium. On the other hand, lowering medium [Na+] to 50 or 75 mm resulted in reduced intracellular [Na+] and [K+]. The increased sensitivity of virus replication to guanidine in the presence of low Na+ medium could be abolished with excess K+ in such medium. Excess K+ in low Na+ medium restored intracellular [Na+] and reduced guanidine uptake. Thus, the increased sensitivity of poliovirus replication to guanidine in the presence of low Na+ medium correlated with reduced intracellular [Na+] and [K+] and elevated guanidine uptake.,
language=,
type=