RT Journal Article SR Electronic(1) A1 Race, Richard E. A1 Fadness, Laverne H. A1 Chesebro, BruceYR 1987 T1 Characterization of Scrapie Infection in Mouse Neuroblastoma Cells JF Journal of General Virology, VO 68 IS 5 SP 1391 OP 1399 DO https://doi.org/10.1099/0022-1317-68-5-1391 PB Microbiology Society, SN 1465-2099, AB Summary A mouse neuroblastoma cell line was successfully infected with scrapie agent. Agent derived from infected mouse brain or spleen infected cultures. However, agent from infected hamsters did not infect mouse cell cultures, suggesting that species specificity influenced the infection process in vitro. Positive cultures supported scrapie replication for as many as 47 passages in vitro. Agent was shown to be cell-associated and between 631 and 7943 unselected culture cells constituted 1 mouse LD50. However, fluctuation analysis indicated that only one of 144 cells in unselected cultures was actually infected. Thus, agent was confined to a small percentage of cells and only 4·4 to 55·1 positive cells were needed to confer a mouse LD50., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-68-5-1391