1887

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Volume 68, Issue 9

Immunoaffinity Purification of a 72K Early Antigen of Human Cytomegalovirus: Analysis of Humoral and Cell-mediated Immunity to the Purified Polypeptide Free

Abstract

Summary

An early antigen of human cytomegalovirus (HCMV) was purified from infected cells as a 72K polypeptide by immunoaffinity chromatography using a monoclonal antibody. It was located in both nucleus and cytoplasm, and was non-glycosylated and undetectable on the surface of infected cells. Known seropositive subjects had antibody against the purified protein and it elicited proliferative T cell responses in 10 of 16 subjects. Five of 14 T cell lines established in response to the purified protein were predominantly CD8 and of these two showed major histocompatibility complex- restricted cytotoxicity against HCMV-infected cells. This provides further evidence that antigens expressed at early times may be targets for the immune response during persistent HCMV infection.

  • Received:
  • Accepted:
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Keyword(s): CMV and human|early antigens|T cells
© Society for General Microbiology 1987
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1987-09-01
2024-04-20
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Immunoaffinity Purification of a 72K Early Antigen of Human Cytomegalovirus: Analysis of Humoral and Cell-mediated Immunity to the Purified Polypeptide
J. Gen. Virol. 68, 2371 (1987); https://doi.org/10.1099/0022-1317-68-9-2371
/content/journal/jgv/10.1099/0022-1317-68-9-2371
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