1887

Journal of General Virology header logo

Volume 69, Issue 10

Bluetongue Virus Genome Remains Stable throughout Prolonged Infection of Cattle Free

Abstract

Summary

Infection of three calves with a highly plaque-purified strain of bluetongue virus (BTV) resulted in prolonged infections, during which virus and neutralizing antibodies co-circulated in peripheral blood. Oligonucleotide fingerprint analyses of the original challenge virus and of the final virus isolate obtained from each calf demonstrated the BTV genome to remain stable throughout prolonged infection as no differences in fingerprint patterns were detected. Six neutralizing monoclonal antibodies (MAbs), and a polyclonal rabbit antiserum, were produced against the challenge virus. This panel of MAbs recognized at least two distinct neutralizing epitopes as demonstrated by immune precipitation. Neutralizing epitopes remained stable through the prolonged infections, as all MAbs and the polyclonal rabbit antiserum neutralized the challenge virus and the final calf isolates to equivalent titres. These results suggest that antigenic drift is not the mechanism by which BTV is able to persist in cattle in spite of a strong humoral immune response.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): BTV , monoclonal antibodies and viraemia
© Society for General Microbiology 1988
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-10-2629
1988-10-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/10/JV0690102629.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-10-2629&mimeType=html&fmt=ahah

References

  1. APPLETON J. A., LETCHWORTH G. J. 1983; Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124:286–299
     [Google Scholar]
  2. BOWNE J. G., LUEDKE A. J., JOCHIM M. M., METCALF H. E. 1968; Bluetongue disease in cattle. Journal of the American Veterinary Medical Association 153:662–668
     [Google Scholar]
  3. CARTER P. B., BEEGLE K. H., GEBHARD D. H. 1986 Monoclonal antibodies: clinical uses and potential. Veterinary Clinics of North America: Small Animal Practice 161171–1179 Edited by Ford R. B. Philadelphia: W.B. Sanders;
     [Google Scholar]
  4. CLEWLEY J., GENTSCH J., BISHOP D. H. L. 1977; Three unique viral RNA species of snowshoe hare and La Crosse Bunyaviruses. Journal of Virology 22:459–468
     [Google Scholar]
  5. COLLISSON E., ROY P. 1983; Analysis of the genomes of bluetongue virus serotype 10 vaccines and a recent BTV-10 isolate from Washington. American Journal of Veterinary Research 44:235–237
     [Google Scholar]
  6. DUTOIT R. M. 1962; The role played by bovines in the transmission of bluetongue in sheep. Journal of the South African Veterinary Medical Association 33:483–490
     [Google Scholar]
  7. EMMONS R. W., OSHIRO L. S., JOHNSON H. N., LENNETTE E. H. 1972; Intra-erythrocytic location of Colorado tick fever virus. Journal of General Virology 17:185–195
     [Google Scholar]
  8. GOTO Y., KUROGI H., INABA Y., MATUMOTO M. 1986; Sequential isolation of rotavirus from individual calves. Veterinary Microbiology 11:177–184
     [Google Scholar]
  9. HUISMANS H., CLOETE M. 1987; A comparison of different cloned bluetongue virus genome segments as probes for the detection of virus-specific RNA. Virology 158:373–380
     [Google Scholar]
  10. HUISMANS H., ERASMUS B. J. 1981; Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Research 48:51–58
     [Google Scholar]
  11. HUISMANS H., CLOETE M., LE ROUX A. 1987; The genetic relatedness of a number of individual cognate genes of viruses in the bluetongue and closely related serogroups. Virology 161:421–428
     [Google Scholar]
  12. HYSLOP N. ST. G., FAGG R. H. 1965; Isolation of variants during passage of a strain of foot and mouth disease virus in partly immunized cattle. Journal of Hygiene 63:357–368
     [Google Scholar]
  13. KAHLON J., SUGIYAMA K., ROY P. 1983; Molecular basis of bluetongue virus neutralization. Journal of Virology 48:627–632
     [Google Scholar]
  14. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  15. LAVER W. G., WEBSTER R. G. 1979; Ecology of influenza viruses in lower mammals and birds. British Medical Bulletin 35:29–33
     [Google Scholar]
  16. LETCHWORTH G. J., APPLETON J. A. 1983; Heterogeneity of neutralization-related epitopes within a bluetongue virus serotype. Virology 124:300–307
     [Google Scholar]
  17. LUEDKE A. J. 1970; Distribution of virus in blood components during viremia of bluetongue. Proceedings of the Annual Meeting of the United States Animal Health Association 74:9–21
     [Google Scholar]
  18. MACLACHLAN N. J., FULLER F. J. 1986; Genetic stability in calves of a single strain of bluetongue virus. American Journal of Veterinary Research 47:762–764
     [Google Scholar]
  19. MACLACHLAN N. J., THOMPSON J. 1985; Bluetongue virus-induced interferon in cattle. American Journal of Veterinary Research 46:1238–1241
     [Google Scholar]
  20. MACLACHLAN N. J., SCHORE C. E., OSBURN B. I. 1984; Antiviral responses of bluetongue virus-inoculated bovine fetuses and their dams. American Journal of Veterinary Research 45:1469–1473
     [Google Scholar]
  21. MACLACHLAN N. J., HEIDNER H. W., FULLER F. J. 1987; Humoral immune response of calves to bluetongue virus infection. American Journal of Veterinary Research 48:1031–1035
     [Google Scholar]
  22. MERTENS P. P. C, BROWN F., SANGAR D. V. 1984; Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology 135:207–217
     [Google Scholar]
  23. MERTENS P. P. C, PEDLEY S., COWLEY J., BURROUGHS J. N. 1987; A comparison of six different bluetongue virus isolates by cross-hybridization of the dsRNA genome segments. Virology 161:438–447
     [Google Scholar]
  24. MITCHELL K. F., FUHRER J. P., STEPLEWSKI Z., KOPROWSKI H. 1980; Biochemical characterization of human melanoma cell surfaces: dissection with monoclonal antibodies. Proceedings of the National Academy of Sciences, U.S.A 77:7287–7291
     [Google Scholar]
  25. MURAKAMI V., NISHIOKA N., WATANABE T., KUNIYASU C. 1986; Prolonged excretion and failure of cross-protection between distinct serotypes of bovine rotavirus. Veterinary Microbiology 12:7–14
     [Google Scholar]
  26. MURPHY F. A., BORDEN E. C, SHOPE R. E., HARRISON A. 1971; Physicochemical and morphological relationships of some arthropod-borne viruses to bluetongue virus—a new taxonomie group. Electron microscopic studies. Journal of General Virology 13:273–288
     [Google Scholar]
  27. NEVILL E. M. 1971; Cattle and culicoides biting midges as possible over-wintering hosts of bluetongue virus. Onderstepoort Journal of Veterinary Research 38:65–72
     [Google Scholar]
  28. RAO C. D., ROY P. 1983; Genetic variation of bluetongue virus serotype 11 isolated from host (sheep) and vector (Culicoides variipennis) at the same site. American Journal of Veterinary Research 44:911–914
     [Google Scholar]
  29. SAMAL S. K., EL-HUSSEIN A., HOLBROOK F. R., BEATY B. J., RAMIG R. F. 1987a; Mixed infection of Culicoides variipennis with bluetongue virus serotypes 10 and 17: evidence for high frequency reassortment in the vector. Journal of General Virology 68:2319–2329
     [Google Scholar]
  30. SAMAL S. K., LIVINGSTON C. W. JR, MCCONNELL S., RAMIG R. F. 1987b; Analysis of mixed infection of sheep with bluetongue virus serotypes 10 and 17: evidence for genetic reassortment in the vertebrate host. Journal of Virology 61:1086–1091
     [Google Scholar]
  31. STOTT J. L., OBERST R. D., CHANNELL M. B., OSBURN B. I. 1987; Genome segment reassortment between two serotypes of bluetongue virus in a natural host. Journal of Virology 61:2670–2674
     [Google Scholar]
  32. SUGIYAMA K., BISHOP D. H. L., ROY P. 1981; Analyses of the genomes of bluetongue viruses recovered in the United States. I. Oligonucleotide fingerprint studies that indicate the existence of naturally occurring reassortant BTV isolates. Virology 114:210–217
     [Google Scholar]
  33. SUGIYAMA K., BISHOP D. H. L., ROY P. 1982; Analyses of the genomes of bluetongue viruses recovered from different states of the United States and at different times. American Journal of Epidemiology 115:332–347
     [Google Scholar]
  34. VERWOERD D. W., LOUW H., OELLERMANN R. A. 1970; Characterization of bluetongue virus ribonucleic acid. Journal of Virology 5:1–7
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-10-2629
Loading
Bluetongue Virus Genome Remains Stable throughout Prolonged Infection of Cattle
J. Gen. Virol. 69, 2629 (1988); https://doi.org/10.1099/0022-1317-69-10-2629
/content/journal/jgv/10.1099/0022-1317-69-10-2629
/content/journal/jgv/10.1099/0022-1317-69-10-2629
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error