f Characterization of the Genome of Equine Herpesvirus 1 Subtype 2
- Authors: Ann A. Cullinane†, Frazer J. Rixon, Andrew J. Davison
- J. Gen. Virol., July 1988 69: 1575-1590, doi: 10.1099/0022-1317-69-7-1575
- Subject: Animal
- Published Online:
The genome structure of equine herpesvirus 1 (EHV-1) subtype 2 was shown by electron microscopic studies and restriction endonuclease site mapping to comprise two covalently linked segments (L, 109 kbp; S, 35 kbp). The S segment contains a unique sequence (US) flanked by a substantial inverted repeat (TRS/IRS). Thus, the genome structure of EHV-1 subtype 2 is similar to that published previously for EHV-1 subtype 1, but the two subtypes differ in the occurrences of EcoRI and BamHI restriction sites. Hybridization studies using cloned EHV-1 DNA showed that the genome of EHV-1 subtype 2 is colinear with the genomes of EHV-1 subtype 1 and herpes simplex virus type 1. DNA sequence data for four EHV-1 subtype 2 genes, including one potentially encoding a glycoprotein, were obtained by sequencing a 4574 bp BamHI fragment containing the junction between US and TRS. The genome structure, hybridization and sequence data confirm that EHV-1 subtype 2 is of the alphaherpesvirus lineage.
Present address: The Irish Equine Centre, Johnstown, Naas, Co. Kildare, Ireland.
© Society for General Microbiology 1988 | Published by the Microbiology Society
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