@article{mbs:/content/journal/jgv/10.1099/0022-1317-70-1-125, author = "Nelson, Randy and Adachi, Anne-Marie and Chisholm, Hugh and Misra, Vikram", title = "Temperature-sensitive Mutants of Bovine Herpesvirus Type 1: Mutants Which Make Unaltered Levels of ‘Early’ Glycoproteins but Fail to Synthesize a ‘Late’ Glycoprotein", journal= "Journal of General Virology", year = "1989", volume = "70", number = "1", pages = "125-132", doi = "https://doi.org/10.1099/0022-1317-70-1-125", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-1-125", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "late", keywords = "temperature-sensitive mutants", keywords = "glycoproteins", keywords = "BHV-1", keywords = "early", abstract = "SUMMARY The major glycoproteins of bovine herpesvirus type 1 showed distinct temporal patterns of expression. The glycoproteins GVP 11 and GVP 6 as well as its cleavage products, GVP 11a and GVP 16, were expressed early in the infectious process, whereas GVP 9 was expressed late. Temperature-sensitive mutants were developed and characterized. Mutants belonging to two complementation groups were unable to synthesize DNA at 40 °C, the non-permissive temperature. In cells infected with these mutants the late glycoprotein GVP 9 was not synthesized at 40 °C, whereasthe synthesis of the early glycoproteins GVP 11 and GVP 6 continued at wild-type levels. These studies suggest that the transition from early to late glycoprotein synthesis is linked to viral DNA synthesis.", }